Bidirectional Sex Change Induced by Sex Steroid Implantation in the Hermaphrodite Fish, Pseudolabrus sieboldi

Kohei Ohta, Mami Sakai, Jittendra Kumar Sundaray, Takeshi Kitano, Tatsusuke Takeda, Akihiko Yamaguchi, Michiya Matsuyama

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Sex steroids have been suggested to be involved in gonadal sex change in hermaphrodite fish. Aromatase, the enzyme that catalyzes the conversion of androgens into estrogens, is a principal enzyme regulating gonadal sex. However, the detailed functions of each steroid hormone remain to be evaluated. Recent studies have demonstrated that estradiol-17β (E2) is synthesized via estrone (E1) in some hermaphrodite species. On the other hand, 11-ketotestosterone (11KT) is produced in the testis via testosterone (T). In this study, we hypothesized that E1 and T are also involved in the sex change as precursors for E2 and 11KT, respectively. We implanted females of the wrasse, Pseudolabrus sieboldi, with T and 11KT, and males with E1 and E2, by use of sustained-release capsules. In females, testicular tissues and body color change were observed after androgen administration, in which 11KT was more effective than T. In contrast, after estrogen administration, the gonads of males contained oocytes. In females, the administration of T and 11KT resulted in reduced serum E2 levels. Conversely, serum 11KT levels decreased in the E1- and E2-treated males. Thus, we successfully induced bidirectional sex change in the gonad by estrogen and androgen administration in vivo. Moreover, this study raises the possibility that E1 and T are involved in the sex change as precursors for E2 and 11KT, respectively.

Original languageEnglish
Pages (from-to)552-560
Number of pages9
JournalJournal of Experimental Zoology Part A: Ecological Genetics and Physiology
Volume317
Issue number9
DOIs
Publication statusPublished - Nov 1 2012

Fingerprint

hermaphrodite
sex reversal
steroid
steroids
androgen
Fishes
androgens
Steroids
estrogens
gender
fish
gonads
serum
Androgens
Estrogens
enzyme
Gonads
estrone
Labridae
testosterone

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Animal Science and Zoology
  • Molecular Biology
  • Genetics

Cite this

Bidirectional Sex Change Induced by Sex Steroid Implantation in the Hermaphrodite Fish, Pseudolabrus sieboldi. / Ohta, Kohei; Sakai, Mami; Sundaray, Jittendra Kumar; Kitano, Takeshi; Takeda, Tatsusuke; Yamaguchi, Akihiko; Matsuyama, Michiya.

In: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, Vol. 317, No. 9, 01.11.2012, p. 552-560.

Research output: Contribution to journalArticle

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