Bile acid is a host factor that regulates the composition of the cecal microbiota in rats

K. B.M.Saiful Islam, Satoru Fukiya, Masahito Hagio, Nobuyuki Fujii, Satoshi Ishizuka, Tadasuke Ooka, Yoshitoshi Ogura, Tetsuya Hayashi, Atsushi Yokota

Research output: Contribution to journalArticle

330 Citations (Scopus)

Abstract

Background & Aims: Alterations in the gastrointestinal microbiota have been associated with metabolic diseases. However, little is known about host factors that induce changes in gastrointestinal bacterial populations. We investigated the role of bile acids in this process because of their strong antimicrobial activities, specifically the effects of cholic acid administration on the composition of the gut microbiota in a rat model. Methods: Rats were fed diets supplemented with different concentrations of cholic acid for 10 days. We used 16S ribosomal RNA gene clone library sequencing and fluorescence in situ hybridization to characterize the composition of the cecal microbiota of the different diet groups. Bile acids in feces, organic acids in cecal contents, and some blood parameters were also analyzed. Results: Administration of cholic acid induced phylum-level alterations in the composition of the gut microbiota; Firmicutes predominated at the expense of Bacteroidetes. Cholic acid feeding simplified the composition of the microbiota, with outgrowth of several bacteria in the classes Clostridia and Erysipelotrichi. Externally administered cholic acid was efficiently transformed into deoxycholic acid by a bacterial 7α-dehydroxylation reaction. Serum levels of adiponectin decreased significantly in rats given the cholic acid diet. Conclusions: Cholic acid regulates the composition of gut microbiota in rats, inducing similar changes to those induced by high-fat diets. These findings improve our understanding of the relationship between metabolic diseases and the composition of the gastrointestinal microbiota.

Original languageEnglish
Pages (from-to)1773-1781
Number of pages9
JournalGastroenterology
Volume141
Issue number5
DOIs
Publication statusPublished - Jan 1 2011
Externally publishedYes

Fingerprint

Cholic Acid
Microbiota
Bile Acids and Salts
Metabolic Diseases
Diet
16S Ribosomal RNA
Bacteroidetes
Deoxycholic Acid
Clostridium
Adiponectin
High Fat Diet
Fluorescence In Situ Hybridization
Gene Library
rRNA Genes
Feces
Clone Cells
Gastrointestinal Microbiome
Bacteria
Acids
Serum

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

Cite this

Islam, K. B. M. S., Fukiya, S., Hagio, M., Fujii, N., Ishizuka, S., Ooka, T., ... Yokota, A. (2011). Bile acid is a host factor that regulates the composition of the cecal microbiota in rats. Gastroenterology, 141(5), 1773-1781. https://doi.org/10.1053/j.gastro.2011.07.046

Bile acid is a host factor that regulates the composition of the cecal microbiota in rats. / Islam, K. B.M.Saiful; Fukiya, Satoru; Hagio, Masahito; Fujii, Nobuyuki; Ishizuka, Satoshi; Ooka, Tadasuke; Ogura, Yoshitoshi; Hayashi, Tetsuya; Yokota, Atsushi.

In: Gastroenterology, Vol. 141, No. 5, 01.01.2011, p. 1773-1781.

Research output: Contribution to journalArticle

Islam, KBMS, Fukiya, S, Hagio, M, Fujii, N, Ishizuka, S, Ooka, T, Ogura, Y, Hayashi, T & Yokota, A 2011, 'Bile acid is a host factor that regulates the composition of the cecal microbiota in rats', Gastroenterology, vol. 141, no. 5, pp. 1773-1781. https://doi.org/10.1053/j.gastro.2011.07.046
Islam, K. B.M.Saiful ; Fukiya, Satoru ; Hagio, Masahito ; Fujii, Nobuyuki ; Ishizuka, Satoshi ; Ooka, Tadasuke ; Ogura, Yoshitoshi ; Hayashi, Tetsuya ; Yokota, Atsushi. / Bile acid is a host factor that regulates the composition of the cecal microbiota in rats. In: Gastroenterology. 2011 ; Vol. 141, No. 5. pp. 1773-1781.
@article{eb091bae31d34f45a3124a09b4f6a5aa,
title = "Bile acid is a host factor that regulates the composition of the cecal microbiota in rats",
abstract = "Background & Aims: Alterations in the gastrointestinal microbiota have been associated with metabolic diseases. However, little is known about host factors that induce changes in gastrointestinal bacterial populations. We investigated the role of bile acids in this process because of their strong antimicrobial activities, specifically the effects of cholic acid administration on the composition of the gut microbiota in a rat model. Methods: Rats were fed diets supplemented with different concentrations of cholic acid for 10 days. We used 16S ribosomal RNA gene clone library sequencing and fluorescence in situ hybridization to characterize the composition of the cecal microbiota of the different diet groups. Bile acids in feces, organic acids in cecal contents, and some blood parameters were also analyzed. Results: Administration of cholic acid induced phylum-level alterations in the composition of the gut microbiota; Firmicutes predominated at the expense of Bacteroidetes. Cholic acid feeding simplified the composition of the microbiota, with outgrowth of several bacteria in the classes Clostridia and Erysipelotrichi. Externally administered cholic acid was efficiently transformed into deoxycholic acid by a bacterial 7α-dehydroxylation reaction. Serum levels of adiponectin decreased significantly in rats given the cholic acid diet. Conclusions: Cholic acid regulates the composition of gut microbiota in rats, inducing similar changes to those induced by high-fat diets. These findings improve our understanding of the relationship between metabolic diseases and the composition of the gastrointestinal microbiota.",
author = "Islam, {K. B.M.Saiful} and Satoru Fukiya and Masahito Hagio and Nobuyuki Fujii and Satoshi Ishizuka and Tadasuke Ooka and Yoshitoshi Ogura and Tetsuya Hayashi and Atsushi Yokota",
year = "2011",
month = "1",
day = "1",
doi = "10.1053/j.gastro.2011.07.046",
language = "English",
volume = "141",
pages = "1773--1781",
journal = "Gastroenterology",
issn = "0016-5085",
publisher = "W.B. Saunders Ltd",
number = "5",

}

TY - JOUR

T1 - Bile acid is a host factor that regulates the composition of the cecal microbiota in rats

AU - Islam, K. B.M.Saiful

AU - Fukiya, Satoru

AU - Hagio, Masahito

AU - Fujii, Nobuyuki

AU - Ishizuka, Satoshi

AU - Ooka, Tadasuke

AU - Ogura, Yoshitoshi

AU - Hayashi, Tetsuya

AU - Yokota, Atsushi

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Background & Aims: Alterations in the gastrointestinal microbiota have been associated with metabolic diseases. However, little is known about host factors that induce changes in gastrointestinal bacterial populations. We investigated the role of bile acids in this process because of their strong antimicrobial activities, specifically the effects of cholic acid administration on the composition of the gut microbiota in a rat model. Methods: Rats were fed diets supplemented with different concentrations of cholic acid for 10 days. We used 16S ribosomal RNA gene clone library sequencing and fluorescence in situ hybridization to characterize the composition of the cecal microbiota of the different diet groups. Bile acids in feces, organic acids in cecal contents, and some blood parameters were also analyzed. Results: Administration of cholic acid induced phylum-level alterations in the composition of the gut microbiota; Firmicutes predominated at the expense of Bacteroidetes. Cholic acid feeding simplified the composition of the microbiota, with outgrowth of several bacteria in the classes Clostridia and Erysipelotrichi. Externally administered cholic acid was efficiently transformed into deoxycholic acid by a bacterial 7α-dehydroxylation reaction. Serum levels of adiponectin decreased significantly in rats given the cholic acid diet. Conclusions: Cholic acid regulates the composition of gut microbiota in rats, inducing similar changes to those induced by high-fat diets. These findings improve our understanding of the relationship between metabolic diseases and the composition of the gastrointestinal microbiota.

AB - Background & Aims: Alterations in the gastrointestinal microbiota have been associated with metabolic diseases. However, little is known about host factors that induce changes in gastrointestinal bacterial populations. We investigated the role of bile acids in this process because of their strong antimicrobial activities, specifically the effects of cholic acid administration on the composition of the gut microbiota in a rat model. Methods: Rats were fed diets supplemented with different concentrations of cholic acid for 10 days. We used 16S ribosomal RNA gene clone library sequencing and fluorescence in situ hybridization to characterize the composition of the cecal microbiota of the different diet groups. Bile acids in feces, organic acids in cecal contents, and some blood parameters were also analyzed. Results: Administration of cholic acid induced phylum-level alterations in the composition of the gut microbiota; Firmicutes predominated at the expense of Bacteroidetes. Cholic acid feeding simplified the composition of the microbiota, with outgrowth of several bacteria in the classes Clostridia and Erysipelotrichi. Externally administered cholic acid was efficiently transformed into deoxycholic acid by a bacterial 7α-dehydroxylation reaction. Serum levels of adiponectin decreased significantly in rats given the cholic acid diet. Conclusions: Cholic acid regulates the composition of gut microbiota in rats, inducing similar changes to those induced by high-fat diets. These findings improve our understanding of the relationship between metabolic diseases and the composition of the gastrointestinal microbiota.

UR - http://www.scopus.com/inward/record.url?scp=80054862011&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80054862011&partnerID=8YFLogxK

U2 - 10.1053/j.gastro.2011.07.046

DO - 10.1053/j.gastro.2011.07.046

M3 - Article

VL - 141

SP - 1773

EP - 1781

JO - Gastroenterology

JF - Gastroenterology

SN - 0016-5085

IS - 5

ER -