Binding of c-Myb to the core sequence of the CD4 promoter

Keiichi Nakayama, Rie Yamamoto, Shunsuke Ishil, Hiromitsu Nakauchl

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We identified a regulatory region of the mouse CD4 promoter by both in vivoand in vitro analysis. The results of transient transfection assays indicated that the dominant transcription activating element within the CD4 promoter is located at -82 to -42. Elimination of this element, by linear deletion or specific mutation, significantly reduced transcriptional activity from this promoter. DNase I footprinting and gel mobility shift assays confirmed that the region -90 to -64 acts as the binding site of a specific nuclear factor, designated NF-CD4. In this region, an 11 bp core motif (CAACAACTGGG; -82 to -72) was found to be sufficient for the binding and transcriptional activation of the NF-CD4. This motif contains a consensus sequence for binding of c-Myb and proteins with helix-loop-helix structures. Indeed, bacterially-synthesized c-Myb specifically binds to this motif for NF-CD4. Furthermore, binding of NF-CD4 to the promoter region was specifically inhibited by the addition of anti-Myb antibodies. The evidence strongly suggests that c-Myb binds, In a sequence-specific fashion, to the core region of the CD4 promoter defined by functional assays and that this proto-oncogene product appears to play a role in the complex regulation of CD4 gene expression during T cell development.

Original languageEnglish
Pages (from-to)817-824
Number of pages8
JournalInternational Immunology
Volume5
Issue number8
DOIs
Publication statusPublished - Aug 1 1993
Externally publishedYes

Fingerprint

Genetic Promoter Regions
Proto-Oncogene Proteins c-myb
Proto-Oncogenes
Nucleic Acid Regulatory Sequences
Deoxyribonuclease I
Oncogene Proteins
Consensus Sequence
Gene Expression Regulation
Electrophoretic Mobility Shift Assay
Transcriptional Activation
Transfection
Anti-Idiotypic Antibodies
Gels
Binding Sites
T-Lymphocytes
Mutation
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Binding of c-Myb to the core sequence of the CD4 promoter. / Nakayama, Keiichi; Yamamoto, Rie; Ishil, Shunsuke; Nakauchl, Hiromitsu.

In: International Immunology, Vol. 5, No. 8, 01.08.1993, p. 817-824.

Research output: Contribution to journalArticle

Nakayama, Keiichi ; Yamamoto, Rie ; Ishil, Shunsuke ; Nakauchl, Hiromitsu. / Binding of c-Myb to the core sequence of the CD4 promoter. In: International Immunology. 1993 ; Vol. 5, No. 8. pp. 817-824.
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