TY - JOUR
T1 - Binding of lectins to DNA micro-assemblies
T2 - Modification of nucleo-cages with lactose-conjugated psoralen
AU - Kim, Kwonil
AU - Matsuura, Kazunori
AU - Kimizuka, Nobuo
N1 - Funding Information:
We thank Professor I. Hamachi (Kyoto University) for the use of the Carl Zeiss LSM 510. This study was financially supported by research grants from the Association for the Progress of New Chemistry, Grant-in-Aid for Scientific Research (A) (16205028) from the Japan Society for the Promotion of Science, and by a Grant for 21st Century COE Program from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2007/6/15
Y1 - 2007/6/15
N2 - Spherical DNA micro-assemblies appended with lactose units (Lactose-nucleo-cages, Lac-NC) are newly developed. DNA spherical assemblies self-assembled from suitably designed three oligodeoxyribonucleotides (ODNs) 1-3 were cross-linked by lactose-conjugated psoralen derivative 4. Confocal laser scanning fluorescence microscopy (CLSM) observation of Lac-NC shows that rhodamine labeled peanut lectin (Rho-PNA: a galactose-specific lectin) binds to lactose-modified nucleo-cages with higher affinity compared to that of unmodified nucleo-cages. Binding isotherm experiments indicated that the apparent affinity constant of Rho-PNA to Lac-NC was in the order of 105 M-1.
AB - Spherical DNA micro-assemblies appended with lactose units (Lactose-nucleo-cages, Lac-NC) are newly developed. DNA spherical assemblies self-assembled from suitably designed three oligodeoxyribonucleotides (ODNs) 1-3 were cross-linked by lactose-conjugated psoralen derivative 4. Confocal laser scanning fluorescence microscopy (CLSM) observation of Lac-NC shows that rhodamine labeled peanut lectin (Rho-PNA: a galactose-specific lectin) binds to lactose-modified nucleo-cages with higher affinity compared to that of unmodified nucleo-cages. Binding isotherm experiments indicated that the apparent affinity constant of Rho-PNA to Lac-NC was in the order of 105 M-1.
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U2 - 10.1016/j.bmc.2007.03.043
DO - 10.1016/j.bmc.2007.03.043
M3 - Article
C2 - 17433697
AN - SCOPUS:34247564539
SN - 0968-0896
VL - 15
SP - 4311
EP - 4317
JO - Bioorganic and Medicinal Chemistry
JF - Bioorganic and Medicinal Chemistry
IS - 12
ER -