Binding of lectins to DNA micro-assemblies: Modification of nucleo-cages with lactose-conjugated psoralen

Kwonil Kim, Kazunori Matsuura, Nobuo Kimizuka

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Spherical DNA micro-assemblies appended with lactose units (Lactose-nucleo-cages, Lac-NC) are newly developed. DNA spherical assemblies self-assembled from suitably designed three oligodeoxyribonucleotides (ODNs) 1-3 were cross-linked by lactose-conjugated psoralen derivative 4. Confocal laser scanning fluorescence microscopy (CLSM) observation of Lac-NC shows that rhodamine labeled peanut lectin (Rho-PNA: a galactose-specific lectin) binds to lactose-modified nucleo-cages with higher affinity compared to that of unmodified nucleo-cages. Binding isotherm experiments indicated that the apparent affinity constant of Rho-PNA to Lac-NC was in the order of 105 M-1.

Original languageEnglish
Pages (from-to)4311-4317
Number of pages7
JournalBioorganic and Medicinal Chemistry
Volume15
Issue number12
DOIs
Publication statusPublished - Jun 15 2007

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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