Bioactivity of high-molecular-weight constituents of Cistanche tubulosa

Shinjiro Maruyama, Taiki Akasaka, Koji Yamada, Hirofumi Tachibana

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cistanche tubulosa (Schrenk) R. Wight is traditionally eaten in Hotan in Xinjiang Uygur Autonomous Region, an area known for longevity and oasis in the Takla-Makan Desert. In this study, we investigated whether high-molecula-weight constituents of C. tubulosa have the same effects on human cell lines as we previously reported for C. salsa. As per results, C. tubulosa dialysate (CTD) prepared using a 3,500-Da molecular weight cut-off dialysis membrane enhanced IgM production in B-cell line BALL-1 and IgG production in B-cell line HMy-2, induced cell proliferation in BALL-1 and T-cell line Jurkat, and oppositely inhibited cell proliferation in B-cell line Namalwa. Using gel filtration chromatography, we found that, similar to C. salsa, the two activities (i.e., enhancement of IgM production and growth inhibition) were concentrated in different fractions. These results suggest that C. tubulosa contains high-molecular-weight constituents having immunomodulatory and direct anti-cancer effects, which seem to contribute to the longevity.

Original languageEnglish
Pages (from-to)913-921
Number of pages9
JournalPharmacologyonline
Volume1
Publication statusPublished - 2009

Fingerprint

Cistanche
Molecular Weight
Cell Line
B-Lymphocytes
Immunoglobulin M
Cell Proliferation
Dialysis Solutions
Gel Chromatography
Dialysis
Immunoglobulin G
T-Lymphocytes
Weights and Measures
Membranes
Growth

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

Cite this

Bioactivity of high-molecular-weight constituents of Cistanche tubulosa. / Maruyama, Shinjiro; Akasaka, Taiki; Yamada, Koji; Tachibana, Hirofumi.

In: Pharmacologyonline, Vol. 1, 2009, p. 913-921.

Research output: Contribution to journalArticle

Maruyama, Shinjiro ; Akasaka, Taiki ; Yamada, Koji ; Tachibana, Hirofumi. / Bioactivity of high-molecular-weight constituents of Cistanche tubulosa. In: Pharmacologyonline. 2009 ; Vol. 1. pp. 913-921.
@article{bb8cbc36f42c44929e8ca26132c13f37,
title = "Bioactivity of high-molecular-weight constituents of Cistanche tubulosa",
abstract = "Cistanche tubulosa (Schrenk) R. Wight is traditionally eaten in Hotan in Xinjiang Uygur Autonomous Region, an area known for longevity and oasis in the Takla-Makan Desert. In this study, we investigated whether high-molecula-weight constituents of C. tubulosa have the same effects on human cell lines as we previously reported for C. salsa. As per results, C. tubulosa dialysate (CTD) prepared using a 3,500-Da molecular weight cut-off dialysis membrane enhanced IgM production in B-cell line BALL-1 and IgG production in B-cell line HMy-2, induced cell proliferation in BALL-1 and T-cell line Jurkat, and oppositely inhibited cell proliferation in B-cell line Namalwa. Using gel filtration chromatography, we found that, similar to C. salsa, the two activities (i.e., enhancement of IgM production and growth inhibition) were concentrated in different fractions. These results suggest that C. tubulosa contains high-molecular-weight constituents having immunomodulatory and direct anti-cancer effects, which seem to contribute to the longevity.",
author = "Shinjiro Maruyama and Taiki Akasaka and Koji Yamada and Hirofumi Tachibana",
year = "2009",
language = "English",
volume = "1",
pages = "913--921",
journal = "Pharmacologyonline",
issn = "1827-8620",
publisher = "SILAE - Societa Italo-Latinoamericana di Etnomedicina",

}

TY - JOUR

T1 - Bioactivity of high-molecular-weight constituents of Cistanche tubulosa

AU - Maruyama, Shinjiro

AU - Akasaka, Taiki

AU - Yamada, Koji

AU - Tachibana, Hirofumi

PY - 2009

Y1 - 2009

N2 - Cistanche tubulosa (Schrenk) R. Wight is traditionally eaten in Hotan in Xinjiang Uygur Autonomous Region, an area known for longevity and oasis in the Takla-Makan Desert. In this study, we investigated whether high-molecula-weight constituents of C. tubulosa have the same effects on human cell lines as we previously reported for C. salsa. As per results, C. tubulosa dialysate (CTD) prepared using a 3,500-Da molecular weight cut-off dialysis membrane enhanced IgM production in B-cell line BALL-1 and IgG production in B-cell line HMy-2, induced cell proliferation in BALL-1 and T-cell line Jurkat, and oppositely inhibited cell proliferation in B-cell line Namalwa. Using gel filtration chromatography, we found that, similar to C. salsa, the two activities (i.e., enhancement of IgM production and growth inhibition) were concentrated in different fractions. These results suggest that C. tubulosa contains high-molecular-weight constituents having immunomodulatory and direct anti-cancer effects, which seem to contribute to the longevity.

AB - Cistanche tubulosa (Schrenk) R. Wight is traditionally eaten in Hotan in Xinjiang Uygur Autonomous Region, an area known for longevity and oasis in the Takla-Makan Desert. In this study, we investigated whether high-molecula-weight constituents of C. tubulosa have the same effects on human cell lines as we previously reported for C. salsa. As per results, C. tubulosa dialysate (CTD) prepared using a 3,500-Da molecular weight cut-off dialysis membrane enhanced IgM production in B-cell line BALL-1 and IgG production in B-cell line HMy-2, induced cell proliferation in BALL-1 and T-cell line Jurkat, and oppositely inhibited cell proliferation in B-cell line Namalwa. Using gel filtration chromatography, we found that, similar to C. salsa, the two activities (i.e., enhancement of IgM production and growth inhibition) were concentrated in different fractions. These results suggest that C. tubulosa contains high-molecular-weight constituents having immunomodulatory and direct anti-cancer effects, which seem to contribute to the longevity.

UR - http://www.scopus.com/inward/record.url?scp=65849471977&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65849471977&partnerID=8YFLogxK

M3 - Article

VL - 1

SP - 913

EP - 921

JO - Pharmacologyonline

JF - Pharmacologyonline

SN - 1827-8620

ER -