Biocatalytic asymmetric phosphorylation of mevalonate

R. Matsumi, C. Hellriegel, B. Schoenenberger, T. Milesi, J. Van Der Oost, R. Wohlgemuth

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The excellent selectivity of the mevalonate kinase-catalyzed phosphorylation of mevalonate simplifies lengthy multi-step routes to (R)-mevalonate-5-phosphate to a one-step biocatalytic reaction, because the phosphate group can be transferred directly and without any additional reaction steps involving introduction and removal of protecting groups. By adjusting the required reaction time for complete conversion, the kinetic resolution of racemic mevalolactone can be easily used for the preparation of (S)-mevalonate and (R)-mevalonate-5-phosphate. A new recombinant mevalonate kinase has been prepared by the expression of the mevalonate kinase gene from the hyperthermophilic archaeon Thermococcus kodakarensis in Escherichia coli and by the subsequent purification. Direct quantitative 31P-NMR kinetic analysis has been utilized to characterize the enantioselectivity of the mevalonate kinase. This method is useful for determining the biocatalyst's utility for the synthesis of enantiomerically pure (R)-mevalonate-5-phosphate as well as for biocatalytic process development. This journal is

Original languageEnglish
Pages (from-to)12989-12994
Number of pages6
JournalRSC Advances
Volume4
Issue number25
DOIs
Publication statusPublished - Mar 17 2014

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mevalonate kinase
Mevalonic Acid
Phosphorylation
Phosphates
Biocatalysts
Kinetics
Enantioselectivity
Escherichia coli
Purification
Genes
Nuclear magnetic resonance
Enzymes
phosphomevalonic acid

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Matsumi, R., Hellriegel, C., Schoenenberger, B., Milesi, T., Van Der Oost, J., & Wohlgemuth, R. (2014). Biocatalytic asymmetric phosphorylation of mevalonate. RSC Advances, 4(25), 12989-12994. https://doi.org/10.1039/c4ra01299b

Biocatalytic asymmetric phosphorylation of mevalonate. / Matsumi, R.; Hellriegel, C.; Schoenenberger, B.; Milesi, T.; Van Der Oost, J.; Wohlgemuth, R.

In: RSC Advances, Vol. 4, No. 25, 17.03.2014, p. 12989-12994.

Research output: Contribution to journalArticle

Matsumi, R, Hellriegel, C, Schoenenberger, B, Milesi, T, Van Der Oost, J & Wohlgemuth, R 2014, 'Biocatalytic asymmetric phosphorylation of mevalonate', RSC Advances, vol. 4, no. 25, pp. 12989-12994. https://doi.org/10.1039/c4ra01299b
Matsumi R, Hellriegel C, Schoenenberger B, Milesi T, Van Der Oost J, Wohlgemuth R. Biocatalytic asymmetric phosphorylation of mevalonate. RSC Advances. 2014 Mar 17;4(25):12989-12994. https://doi.org/10.1039/c4ra01299b
Matsumi, R. ; Hellriegel, C. ; Schoenenberger, B. ; Milesi, T. ; Van Der Oost, J. ; Wohlgemuth, R. / Biocatalytic asymmetric phosphorylation of mevalonate. In: RSC Advances. 2014 ; Vol. 4, No. 25. pp. 12989-12994.
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