Biochemical characterization of an unclassified glutathione S-transferase of Plutella xylostella

Kohji Yamamoto, Aiko Hirowatari, Takahiro Shiotsuki, Naotaka Yamada

Research output: Contribution to journalArticle

Abstract

cDNA encoding an unclassified glutathione S-transferase (GST) of the diamondback moth, Plutella xylostella, was cloned by reverse transcriptase-polymerase chain reaction. The resulting clone was sequenced and the amino acid sequence deduced, revealing 67%-73% identities with unclassified GSTs from other organisms. A recombinant protein was functionally overexpressed in Escherichia coli cells in a soluble form and purified to homogeneity. The enzyme was capable to catalyze the transformation of 1-chloro-2,4-dinitrobenzene and ethacrynic acid with glutathione. A competition assay revealed that GST activity was inhibited by insecticides, suggesting that the enzyme could contribute to insecticide metabolism in the diamondback moth.

Original languageEnglish
Pages (from-to)145-151
Number of pages7
JournalJournal of Pesticide Science
Volume41
Issue number4
DOIs
Publication statusPublished - Jan 1 2016

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Moths
Plutella xylostella
Insecticides
Glutathione Transferase
glutathione transferase
Ethacrynic Acid
Dinitrochlorobenzene
insecticides
Enzymes
ethacrynic acid
Reverse Transcriptase Polymerase Chain Reaction
Recombinant Proteins
Glutathione
Amino Acid Sequence
Complementary DNA
Clone Cells
Escherichia coli
enzymes
recombinant proteins
glutathione

All Science Journal Classification (ASJC) codes

  • Insect Science
  • Health, Toxicology and Mutagenesis

Cite this

Biochemical characterization of an unclassified glutathione S-transferase of Plutella xylostella. / Yamamoto, Kohji; Hirowatari, Aiko; Shiotsuki, Takahiro; Yamada, Naotaka.

In: Journal of Pesticide Science, Vol. 41, No. 4, 01.01.2016, p. 145-151.

Research output: Contribution to journalArticle

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