Biochemical characterization of an unclassified glutathione S-transferase of Plutella xylostella

Kohji Yamamoto, Aiko Hirowatari, Takahiro Shiotsuki, Naotaka Yamada

Research output: Contribution to journalArticlepeer-review

Abstract

cDNA encoding an unclassified glutathione S-transferase (GST) of the diamondback moth, Plutella xylostella, was cloned by reverse transcriptase-polymerase chain reaction. The resulting clone was sequenced and the amino acid sequence deduced, revealing 67%-73% identities with unclassified GSTs from other organisms. A recombinant protein was functionally overexpressed in Escherichia coli cells in a soluble form and purified to homogeneity. The enzyme was capable to catalyze the transformation of 1-chloro-2,4-dinitrobenzene and ethacrynic acid with glutathione. A competition assay revealed that GST activity was inhibited by insecticides, suggesting that the enzyme could contribute to insecticide metabolism in the diamondback moth.

Original languageEnglish
Pages (from-to)145-151
Number of pages7
JournalJournal of Pesticide Science
Volume41
Issue number4
DOIs
Publication statusPublished - 2016

All Science Journal Classification (ASJC) codes

  • Insect Science
  • Health, Toxicology and Mutagenesis

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