Biochemical characterization of liver microsomal, golgi, lysosomal, and serum β-glucuronidases in dibuty1 phosphate-treated rats

Yukio Nishimura, Keitaro Kato, Masaru Himeno

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Abstract

Organophosphate compounds are known to cause the selective release of rat liver microsomal β-glucuronidase into plasma. To investigate the alterations of molecular forms and oligosaccharide moieties of liver β-glucuronidase in organophosphate compound-administered rats, β-glucuronidase was isolated from microsomal, Golgi, lysosomal, and serum fractions. In SDS-polyacry lamide gel electrophoresis, a single polypeptide band was observed on gels in Golgi and serum β-glucuronidases. This result indicated that Golgi and serum β-glucuronidases of treated rats did not undergo post-translational proteolytic processing, in contrast to those in control rat livers. Biochemical characterization of the isolated β-glucuronidases by employing lectin affinity chromatography revealed that interaction of serum and Golgi enzymes with Ricinus communis agglutinin- and wheat germ agglutinin-Sepharose was fairly strong, and that microsomal and lysosomal enzymes were poorly retained on those columns. These results suggested that the serum and Golgi β-glucuronidases are sialoglycoproteins. A clearance study also showed that infused serum β-glucuronidase was slowly cleared from plasma with a half-life of about 60 min, but the asialo-serum enzyme was rapidly cleared with a half-life of about 5 min. These results imply that microsomal β-glucuronidase undergoes extensive modification of the oligosaccharide moieties by terminal glycosyltransferases at the trans Golgi when it is destined for secretion into serum in response to treatment with an organophosphate compound.

Original languageEnglish
Pages (from-to)56-66
Number of pages11
JournalJournal of biochemistry
Volume118
Issue number1
DOIs
Publication statusPublished - Jan 1 1995

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Glucuronidase
Liver
Rats
Phosphates
Serum
Organophosphates
Oligosaccharides
Half-Life
Enzymes
Gels
Rat control
Sialoglycoproteins
Plasmas
Affinity chromatography
Glycosyltransferases
Wheat Germ Agglutinins
Electrophoresis
Affinity Chromatography
Lectins
Sepharose

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Biochemical characterization of liver microsomal, golgi, lysosomal, and serum β-glucuronidases in dibuty1 phosphate-treated rats. / Nishimura, Yukio; Kato, Keitaro; Himeno, Masaru.

In: Journal of biochemistry, Vol. 118, No. 1, 01.01.1995, p. 56-66.

Research output: Contribution to journalArticle

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