Biochemical studies on 2-deoxy-scyllo-inosose, an early intermediate in the biosynthesis of 2-deoxystreptamine ii. Quantitative analysis of 2-deoxy-sc7llo-inosose

Noriaki Yamauchi, Katsumi Kakinuma

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A practical quantitative analysis of 2-deoxy-scyllo-inosose by means of GC-MS selected-ion monitoring (SIM) was exploited in order to assay the enzymatic 2-deoxy-scyllo-inosose formation, the first stage of the 2-deoxystreptamine biosynthesis. Mass spectral fragmentations of 2-deoxy-scyllo-inosose tetra-O-trimethylsilyl (TMS) ether was also investigated by the use of [2,2-2H2]-2-deoxy-scyllo-inosose.

Original languageEnglish
Pages (from-to)767-773
Number of pages7
JournalThe Journal of Antibiotics
Volume45
Issue number5
DOIs
Publication statusPublished - Jan 1 1992
Externally publishedYes

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Enzyme Assays
Ether
Ions
2-deoxyinosose
inosose
2-deoxystreptamine

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

Cite this

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