Biochemical switching device: Monocyclic enzyme system

Masahiro Okamoto, Toshio Sakai, Katsuya Hayashi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The switching characteristics of a monocyclic enzyme system, in which two enzymes share substrates or co‐factors in a cyclic manner, such as, → X1 + B + E1 ⇄ A + E1 + X2 →, → X3 + A + E2 ⇄ B + E2 + X4 → (E1, E2 are enzymes, X1, X3 are substrates, X2, X4 are products, A, B are cofactors), were demonstrated using computer simulations. The detailed mathematical models of biochemically possible cyclic enzyme systems were built up and the effects of rate constants and the effects of initial concentrations of enzymes and cofactors on switching characteristics were discussed. The cyclic enzyme system could function as a switching circuit when the initial concentrations of enzymes or cofactors are over a certain threshold value. Based on the present results, we further discuss the dynamic characteristics of a biochemical reactor system (bioreactor) involving this cyclic enzyme system as a switching controller.

Original languageEnglish
Pages (from-to)527-537
Number of pages11
JournalBiotechnology and Bioengineering
Volume32
Issue number4
DOIs
Publication statusPublished - Aug 5 1988

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Enzymes
Equipment and Supplies
Coenzymes
Switching circuits
Computer Simulation
Substrates
Theoretical Models
Rate constants
Mathematical models
Controllers
Computer simulation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Biochemical switching device : Monocyclic enzyme system. / Okamoto, Masahiro; Sakai, Toshio; Hayashi, Katsuya.

In: Biotechnology and Bioengineering, Vol. 32, No. 4, 05.08.1988, p. 527-537.

Research output: Contribution to journalArticle

Okamoto, Masahiro ; Sakai, Toshio ; Hayashi, Katsuya. / Biochemical switching device : Monocyclic enzyme system. In: Biotechnology and Bioengineering. 1988 ; Vol. 32, No. 4. pp. 527-537.
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