Biodegradation of methylthio-s-triazines by Rhodococcus sp. strain FJ1117YT, and production of the corresponding methylsulfinyl, methylsulfonyl and hydroxy analogues

Kunihiko Fujii, Kazuhiro Takagi, Syuntaro Hiradate, Akio Iwasaki, Naoki Harada

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A novel bacterial strain FJ1117YT was isolated from an enrichment culture with the herbicide simetryn. The isolate was capable of degrading the herbicide supplied as the sole sulfur source in an aquatic batch culture. The strain FJ1117YT was identified as that belonging to Rhodococcus sp. on the basis of comparative morphology, physiological characteristics and comparison of the 16S rRNA gene sequence. The biodegradation pathway of simetryn was established by isolating the methylsulfinyl analogue as the first metabolite and by identification of the methylsulfonyl intermediate and the hydroxy analogue by liquid chromatography-mass spectrometry (LC-MS) and/or nuclear magnetic resonance (NMR) analysis. The results indicate that the methylthio group was progressively oxidised and hydrolysed by the strain FJ1117YT. The same strain is also able to metabolise other methylthio-s-triazines such as ametryn, desmetryn, dimethametryn and prometryn through similar pathways.

Original languageEnglish
Pages (from-to)254-260
Number of pages7
JournalPest Management Science
Volume63
Issue number3
DOIs
Publication statusPublished - Mar 1 2007
Externally publishedYes

Fingerprint

triazines
biodegradation
simetryn
desmetryn
dimethametryn
herbicides
ametryn
prometryn
enrichment culture
liquid chromatography
nuclear magnetic resonance spectroscopy
sulfur
ribosomal RNA
mass spectrometry
metabolites
nucleotide sequences

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Insect Science

Cite this

Biodegradation of methylthio-s-triazines by Rhodococcus sp. strain FJ1117YT, and production of the corresponding methylsulfinyl, methylsulfonyl and hydroxy analogues. / Fujii, Kunihiko; Takagi, Kazuhiro; Hiradate, Syuntaro; Iwasaki, Akio; Harada, Naoki.

In: Pest Management Science, Vol. 63, No. 3, 01.03.2007, p. 254-260.

Research output: Contribution to journalArticle

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