Biodegradation of phenolic environmental pollutants by a surfactant-laccase complex in organic media

Junji Michizoe, Hirofumi Ichinose, Noriho Kamiya, Tatsuo Maruyama, Masahiro Goto

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Oxidative degradation of phenolic environmental pollutants in organic media was investigated using a laccase complexed with surfactants. The catalytic activity of the surfactant-laccase complex in isooctane was markedly enhanced by appropriately adjusting the water content of the reaction medium using reverse micelles. The surfactant-laccase complex showed little activity towards the oxidative reaction of bisphenol A in water-saturated isooctane (i.e., 0.0055% [v/v] water) while effectively catalyzed the same reaction in isooctane containing 4% (v/v) water, which is over the maximum water solubility. The latter system was homogeneous and was only achieved by the aid of reverse micelles. With respect to the oxidation of bisphenol A, two products, 4-isopropylphenol and 4-isopropenylphenol, were identified by gas chromatography-mass spectrometry (GC-MS) analyses, indicating the oxidative degradation of the bis-phenolic structure of bis-phenol A. We also found that the surfactant-laccase complex turned out to handle other environmental pollutants, chlorophenols, by the simultaneous addition of water and a redox mediator into the reaction medium using reverse micelles.

Original languageEnglish
Pages (from-to)642-647
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume99
Issue number6
DOIs
Publication statusPublished - Jan 1 2005

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Laccase
Environmental Pollutants
Biodegradation
Surface-Active Agents
Surface active agents
Micelles
Water
Chlorophenols
Degradation
Phenol
Gas chromatography
Water content
Phenols
Mass spectrometry
Catalyst activity
Solubility
Gas Chromatography-Mass Spectrometry
Oxidation-Reduction
Oxidation
2,2,4-trimethylpentane

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Biodegradation of phenolic environmental pollutants by a surfactant-laccase complex in organic media. / Michizoe, Junji; Ichinose, Hirofumi; Kamiya, Noriho; Maruyama, Tatsuo; Goto, Masahiro.

In: Journal of Bioscience and Bioengineering, Vol. 99, No. 6, 01.01.2005, p. 642-647.

Research output: Contribution to journalArticle

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