Biodistribution and tumor localization of peg-modified dendritic poly(L-lysine) oligonucleotide complexes

Ryohsuke Kurihara, Dakrong Pissuwan, Takeshi Mori, Yoshiki Katayama, Takuro Niidome

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A poly(ethylene glycol) (PEG)-modified dendritic poly(L-lysine) (PEG-WeKG6) containing tryptophan residues in its core was synthesized as an oligonucleotide carrier to tumors after systemic injection. PEG-WeKG6 formed a stable complex with double-stranded deoxyoligonucleotide (ODN). The size and the zeta-potential of the complex were smaller than those of a dendritic poly(L-lysine) without PEG (WeKG6). To study the biodistribution of the complexes in tumor-bearing mice after intravenous injection, the den-drimers and the oligonucleotide were labeled with gadolinium and Cy5, respectively. Our results show that PEG modification of the dendrimer improved the stability of ODN in blood circulation. Effective accumulation of the PEG-WeKG6/ODN complex in the tumor tissue was found 24 h after the injection. These results indicate that PEG-WeKG6 is suitable for forming a complex with any genetic or therapeutic material for efficient delivery to tumors.

Original languageEnglish
Pages (from-to)2369-2380
Number of pages12
JournalJournal of Biomaterials Science, Polymer Edition
Volume23
Issue number18
DOIs
Publication statusPublished - Oct 12 2012

Fingerprint

Oligonucleotides
Polyethylene glycols
Lysine
Tumors
Neoplasms
Dendrimers
Injections
Ethylene Glycol
Blood Circulation
Gadolinium
Intravenous Injections
Tryptophan
Bearings (structural)
Hemodynamics
Zeta potential
Tissue
Therapeutics

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Biodistribution and tumor localization of peg-modified dendritic poly(L-lysine) oligonucleotide complexes. / Kurihara, Ryohsuke; Pissuwan, Dakrong; Mori, Takeshi; Katayama, Yoshiki; Niidome, Takuro.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 23, No. 18, 12.10.2012, p. 2369-2380.

Research output: Contribution to journalArticle

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