TY - JOUR
T1 - Biodistribution and tumor localization of peg-modified dendritic poly(L-lysine) oligonucleotide complexes
AU - Kurihara, Ryohsuke
AU - Pissuwan, Dakrong
AU - Mori, Takeshi
AU - Katayama, Yoshiki
AU - Niidome, Takuro
N1 - Funding Information:
This research was supported by a Grant-in-Aid for Scientific Research (B) (No. 19300172 and 22300158) from the Japan Society for the Promotion of Science (JSPS).
PY - 2012
Y1 - 2012
N2 - A poly(ethylene glycol) (PEG)-modified dendritic poly(L-lysine) (PEG-WeKG6) containing tryptophan residues in its core was synthesized as an oligonucleotide carrier to tumors after systemic injection. PEG-WeKG6 formed a stable complex with double-stranded deoxyoligonucleotide (ODN). The size and the zeta-potential of the complex were smaller than those of a dendritic poly(L-lysine) without PEG (WeKG6). To study the biodistribution of the complexes in tumor-bearing mice after intravenous injection, the den-drimers and the oligonucleotide were labeled with gadolinium and Cy5, respectively. Our results show that PEG modification of the dendrimer improved the stability of ODN in blood circulation. Effective accumulation of the PEG-WeKG6/ODN complex in the tumor tissue was found 24 h after the injection. These results indicate that PEG-WeKG6 is suitable for forming a complex with any genetic or therapeutic material for efficient delivery to tumors.
AB - A poly(ethylene glycol) (PEG)-modified dendritic poly(L-lysine) (PEG-WeKG6) containing tryptophan residues in its core was synthesized as an oligonucleotide carrier to tumors after systemic injection. PEG-WeKG6 formed a stable complex with double-stranded deoxyoligonucleotide (ODN). The size and the zeta-potential of the complex were smaller than those of a dendritic poly(L-lysine) without PEG (WeKG6). To study the biodistribution of the complexes in tumor-bearing mice after intravenous injection, the den-drimers and the oligonucleotide were labeled with gadolinium and Cy5, respectively. Our results show that PEG modification of the dendrimer improved the stability of ODN in blood circulation. Effective accumulation of the PEG-WeKG6/ODN complex in the tumor tissue was found 24 h after the injection. These results indicate that PEG-WeKG6 is suitable for forming a complex with any genetic or therapeutic material for efficient delivery to tumors.
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U2 - 10.1163/156856211X617722
DO - 10.1163/156856211X617722
M3 - Article
C2 - 22244201
AN - SCOPUS:84867204178
SN - 0920-5063
VL - 23
SP - 2369
EP - 2380
JO - Journal of Biomaterials Science, Polymer Edition
JF - Journal of Biomaterials Science, Polymer Edition
IS - 18
ER -