Biodistribution of locally or systemically transplanted osteoblast-like cells

Y. T. Okabe, T. Kondo, K. Mishima, Y. Hayase, K. Kato, M. Mizuno, N. Ishiguro, H. Kitoh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objectives: In order to ensure safety of the cell-based therapy for bone regeneration, we examined in vivo biodistribution of locally or systemically transplanted osteoblast-like cells generated from bone marrow (BM) derived mononuclear cells. Methods: BM cells obtained from a total of 13 Sprague-Dawley (SD) green fluorescent protein transgenic (GFP-Tg) rats were culture-expanded in an osteogenic differentiation medium for three weeks. Osteoblast-like cells were then locally transplanted with collagen scaffolds to the rat model of segmental bone defect. Donor cells were also intravenously infused to the normal Sprague-Dawley (SD) rats for systemic biodistribution. The flow cytometric and histological analyses were performed for cellular tracking after transplantation. Results: Locally transplanted donor cells remained within the vicinity of the transplantation site without migrating to other organs. Systemically administered large amounts of osteoblast-like cells were cleared from various organ tissues within three days of transplantation and did not show any adverse effects in the transplanted rats. Conclusions: We demonstrated a precise assessment of donor cell biodistribution that further augments prospective utility of regenerative cell therapy.

Original languageEnglish
Pages (from-to)76-81
Number of pages6
JournalBone and Joint Research
Volume3
Issue number3
DOIs
Publication statusPublished - Mar 2014
Externally publishedYes

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Osteoblasts
Transplantation
Cell- and Tissue-Based Therapy
Bone Marrow Cells
Transgenic Rats
Bone Regeneration
Green Fluorescent Proteins
Sprague Dawley Rats
Collagen
Safety
Bone and Bones

All Science Journal Classification (ASJC) codes

  • Surgery
  • Orthopedics and Sports Medicine

Cite this

Biodistribution of locally or systemically transplanted osteoblast-like cells. / Okabe, Y. T.; Kondo, T.; Mishima, K.; Hayase, Y.; Kato, K.; Mizuno, M.; Ishiguro, N.; Kitoh, H.

In: Bone and Joint Research, Vol. 3, No. 3, 03.2014, p. 76-81.

Research output: Contribution to journalArticle

Okabe, YT, Kondo, T, Mishima, K, Hayase, Y, Kato, K, Mizuno, M, Ishiguro, N & Kitoh, H 2014, 'Biodistribution of locally or systemically transplanted osteoblast-like cells', Bone and Joint Research, vol. 3, no. 3, pp. 76-81. https://doi.org/10.1302/2046-3758.33.2000257
Okabe, Y. T. ; Kondo, T. ; Mishima, K. ; Hayase, Y. ; Kato, K. ; Mizuno, M. ; Ishiguro, N. ; Kitoh, H. / Biodistribution of locally or systemically transplanted osteoblast-like cells. In: Bone and Joint Research. 2014 ; Vol. 3, No. 3. pp. 76-81.
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AU - Mizuno, M.

AU - Ishiguro, N.

AU - Kitoh, H.

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