Biofertilizer production for agronomic application and evaluation of its symbiotic effectiveness in soybeans

Aung Zaw Htwe, Seinn Moh Moh, Kyi Moe, Takeo Yamakawa

Research output: Contribution to journalArticle

Abstract

This study was conducted to evaluate the effects of Bradyrhizobium japonicum SAY3-7, Bradyrhizobium elkanii BLY3-8, and Streptomyces griseoflavus P4 on the symbiotic effectiveness of soybeans before biofertilizer production, to produce biofertilizer containing the studied three strains (SAY3-7, BLY3-8, and P4), to test the effectiveness of the biofertilizer on soybean varieties, and to assess the varietal effects and interaction effects between variety and biofertilizer on plant growth, nodulation, nitrogen fixation, nutrient absorption, and seed yield. Nitrogen fixation was measured using the acetylene reduction assay and ureide methods. Contents of nutrients (N, P, K, Ca, and Mg) were also measured to calculate their uptakes. In this study, synergistic effects of nitrogen fixation were induced by combined inoculation with SAY3-7, BLY3-8 and P4 in all tested soybean varieties. Therefore, we assumed that an effective biofertilizer could be produced using these effective bacteria (SAY3-7, BLY3-8, and P4). After making biofertilizer using these effective bacteria, packages were stored at 30 C. The populations of the bacteria in the biofertilizer were maintained at a density of 1 × 10 8 colony forming units (cfu) g 1 for P4 and 7 × 10 9 cells g 1 for Bradyrhizobium. Diluting biofertilizer by 10 3 proved more effective for nodulation and nitrogen fixation than other dilution treatments. Moreover, this biofertilizer significantly promoted plant growth, nodulation, nitrogen fixation, nutrient uptakes, and seed yield in Yezin-3 and Yezin-6 soybean varieties. Yezin-6 is a more efficient variety than Yezin-3 for improved plant growth, nodulation, nitrogen fixation, nutrient absorption, and seed yield. Taken together, the application of an effective biofertilizer and the use of an efficient soybean variety can play important roles in promoting plant growth, nodulation, nitrogen fixation, and higher seed yield.

Original languageEnglish
Article number162
JournalAgronomy
Volume9
Issue number4
DOIs
Publication statusPublished - Mar 27 2019

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biofertilizers
soybeans
nitrogen fixation
nodulation
seed yield
plant growth
Streptomyces griseoflavus
bacteria
Bradyrhizobium elkanii
ureides
Bradyrhizobium
Bradyrhizobium japonicum
acetylene reduction
nutrients
nutrient uptake
nutrient content
uptake mechanisms

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science

Cite this

Biofertilizer production for agronomic application and evaluation of its symbiotic effectiveness in soybeans. / Htwe, Aung Zaw; Moh, Seinn Moh; Moe, Kyi; Yamakawa, Takeo.

In: Agronomy, Vol. 9, No. 4, 162, 27.03.2019.

Research output: Contribution to journalArticle

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