Ethyl acetate extracts of Cupressus lusitanica suspension cell cultures were examined for their biological activities (viz., tyrosinase inhibitory, antioxidant, and antimicrobial activities). The extract from elicitor-treated cells showed all of the biological activities, whereas the extract from cultures without elicitor was not bioactive to a discernible extent. The biological activities shown by the cell culture extracts were almost solely accounted for by β-thujaplicin contained in the extracts. These results suggest that the ethyl acetate extract of C. lusitanica cells treated with the elicitor is a valuable bioactive source without isolation or purification of β-thujaplicin.
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