Biological and genetic characteristics of tumor-initiating cells in colon cancer

Keisuke Ieta, Fumiaki Tanaka, Naotsugu Haraguchi, Yoshiaki Kita, Hiroyuki Sakashita, Koshi Mimori, Toshifumi Matsumoto, Hiroshi Inoue, Hiroyuki Kuwano, Masaki Mori

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

Background: Human prominin-1 (PROM1, CD133) was used as a marker to detect stem cells (progenitor cells) and cancer stem cells (tumor-initiating cells) in various tissues. The purpose of this study was to investigate the biological and genetic characteristics of tumor-initiating cells in colon cancer with both in vitro and in vivo analyses. Methods: The CD133 expression of 12 colon cancer cell lines was evaluated. CD133+ cells were isolated by flow cytometry and examined for in vivo tumor formation, in vitro proliferation, colony formation, and invasion ability. Additionally, we used microarray analysis to compare gene expression profiles between CD133+ and CD133- isolated cells. Results: CD133+ cells were found in 5 of 12 colon cancer cell lines. Isolated CD133+ cells from the HT29 colon cancer cell line exhibited a higher tumorigenic potential than CD133 - cells in the in vivo tumor formation assay. Furthermore, it was shown that CD133+ cells are more proliferative and have higher colony-forming and invasive abilities than CD133- cells in vitro. Microarray analysis found differential gene expression correlating with CD133 expression. Conclusions: It was confirmed that CD133+ cells in colon cancer are useful markers for the detection of tumor-initiating cells. Intimate biological and genetic features of CD133+ cells in colon cancer cell lines were also revealed. The biological characteristics of CD133+ cells and differentially expressed genes in these cells will help elucidate more details of tumor-initiating cells in colon cancer.

Original languageEnglish
Pages (from-to)638-648
Number of pages11
JournalAnnals of Surgical Oncology
Volume15
Issue number2
DOIs
Publication statusPublished - Feb 1 2008

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Neoplastic Stem Cells
Colonic Neoplasms
Cell Line
Microarray Analysis
Stem Cells
HT29 Cells
Transcriptome
Neoplasms
Flow Cytometry
Gene Expression

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oncology

Cite this

Biological and genetic characteristics of tumor-initiating cells in colon cancer. / Ieta, Keisuke; Tanaka, Fumiaki; Haraguchi, Naotsugu; Kita, Yoshiaki; Sakashita, Hiroyuki; Mimori, Koshi; Matsumoto, Toshifumi; Inoue, Hiroshi; Kuwano, Hiroyuki; Mori, Masaki.

In: Annals of Surgical Oncology, Vol. 15, No. 2, 01.02.2008, p. 638-648.

Research output: Contribution to journalArticle

Ieta, K, Tanaka, F, Haraguchi, N, Kita, Y, Sakashita, H, Mimori, K, Matsumoto, T, Inoue, H, Kuwano, H & Mori, M 2008, 'Biological and genetic characteristics of tumor-initiating cells in colon cancer', Annals of Surgical Oncology, vol. 15, no. 2, pp. 638-648. https://doi.org/10.1245/s10434-007-9605-3
Ieta, Keisuke ; Tanaka, Fumiaki ; Haraguchi, Naotsugu ; Kita, Yoshiaki ; Sakashita, Hiroyuki ; Mimori, Koshi ; Matsumoto, Toshifumi ; Inoue, Hiroshi ; Kuwano, Hiroyuki ; Mori, Masaki. / Biological and genetic characteristics of tumor-initiating cells in colon cancer. In: Annals of Surgical Oncology. 2008 ; Vol. 15, No. 2. pp. 638-648.
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abstract = "Background: Human prominin-1 (PROM1, CD133) was used as a marker to detect stem cells (progenitor cells) and cancer stem cells (tumor-initiating cells) in various tissues. The purpose of this study was to investigate the biological and genetic characteristics of tumor-initiating cells in colon cancer with both in vitro and in vivo analyses. Methods: The CD133 expression of 12 colon cancer cell lines was evaluated. CD133+ cells were isolated by flow cytometry and examined for in vivo tumor formation, in vitro proliferation, colony formation, and invasion ability. Additionally, we used microarray analysis to compare gene expression profiles between CD133+ and CD133- isolated cells. Results: CD133+ cells were found in 5 of 12 colon cancer cell lines. Isolated CD133+ cells from the HT29 colon cancer cell line exhibited a higher tumorigenic potential than CD133 - cells in the in vivo tumor formation assay. Furthermore, it was shown that CD133+ cells are more proliferative and have higher colony-forming and invasive abilities than CD133- cells in vitro. Microarray analysis found differential gene expression correlating with CD133 expression. Conclusions: It was confirmed that CD133+ cells in colon cancer are useful markers for the detection of tumor-initiating cells. Intimate biological and genetic features of CD133+ cells in colon cancer cell lines were also revealed. The biological characteristics of CD133+ cells and differentially expressed genes in these cells will help elucidate more details of tumor-initiating cells in colon cancer.",
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AU - Tanaka, Fumiaki

AU - Haraguchi, Naotsugu

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AU - Sakashita, Hiroyuki

AU - Mimori, Koshi

AU - Matsumoto, Toshifumi

AU - Inoue, Hiroshi

AU - Kuwano, Hiroyuki

AU - Mori, Masaki

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AB - Background: Human prominin-1 (PROM1, CD133) was used as a marker to detect stem cells (progenitor cells) and cancer stem cells (tumor-initiating cells) in various tissues. The purpose of this study was to investigate the biological and genetic characteristics of tumor-initiating cells in colon cancer with both in vitro and in vivo analyses. Methods: The CD133 expression of 12 colon cancer cell lines was evaluated. CD133+ cells were isolated by flow cytometry and examined for in vivo tumor formation, in vitro proliferation, colony formation, and invasion ability. Additionally, we used microarray analysis to compare gene expression profiles between CD133+ and CD133- isolated cells. Results: CD133+ cells were found in 5 of 12 colon cancer cell lines. Isolated CD133+ cells from the HT29 colon cancer cell line exhibited a higher tumorigenic potential than CD133 - cells in the in vivo tumor formation assay. Furthermore, it was shown that CD133+ cells are more proliferative and have higher colony-forming and invasive abilities than CD133- cells in vitro. Microarray analysis found differential gene expression correlating with CD133 expression. Conclusions: It was confirmed that CD133+ cells in colon cancer are useful markers for the detection of tumor-initiating cells. Intimate biological and genetic features of CD133+ cells in colon cancer cell lines were also revealed. The biological characteristics of CD133+ cells and differentially expressed genes in these cells will help elucidate more details of tumor-initiating cells in colon cancer.

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