Abstract
Pectobacterium carotovorum (Pc) causing potato tuber soft rot uses N-acyl-L-homoserine lactones (AHLs) to control the production of virulence factors via quorum sensing (QS). Some bacteria produce enzymes to inactivate the AHL signals of pathogenic bacteria via a phenomenon known as quorum quenching. One hundred bacterial isolates from potato tubers were screened for AHL-degrading activity using biosensor strain Chromobacterium violaceum CV026. Of these isolates, 20 were able to inactivate AHLs from the pathogenic bacteria in vitro. Of the 20 isolates, 6 attenuated tissue maceration of potato tubers by Pc. Suppression of tuber soft rot was observed even when these isolates were applied 24 h after the pathogen was introduced. Their colonization in tubers was approximately 103-104 cfu/g tuber, 7 days after inoculation. These isolates were identified as Bacillus sp., Variovorax sp., Variovorax paradoxus and Agrobacterium tumefaciens. Four of these isolates showed putative AHL-lactonase activity and provided the most significant protection against Pc. Therefore, AHL-degrading endophytic bacteria can be utilized as a novel biocontrol agent of potato tuber soft rot in Vietnam.
| Original language | English |
|---|---|
| Pages (from-to) | 1921-1927 |
| Number of pages | 7 |
| Journal | Current Science |
| Volume | 115 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Nov 1 2018 |
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Biological control of potato tuber soft rot using N-acyl-L-homoserine lactone-degrading endophytic bacteria. / Ha, Nguyen Thanh; Minh, Tran Quang; Hoi, Pham Xuan; Thanh Thuy, Nguyen Thi; Furuya, Naruto; Long, Hoang Hoa.
In: Current Science, Vol. 115, No. 10, 01.11.2018, p. 1921-1927.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Biological control of potato tuber soft rot using N-acyl-L-homoserine lactone-degrading endophytic bacteria
AU - Ha, Nguyen Thanh
AU - Minh, Tran Quang
AU - Hoi, Pham Xuan
AU - Thanh Thuy, Nguyen Thi
AU - Furuya, Naruto
AU - Long, Hoang Hoa
PY - 2018/11/1
Y1 - 2018/11/1
N2 - Pectobacterium carotovorum (Pc) causing potato tuber soft rot uses N-acyl-L-homoserine lactones (AHLs) to control the production of virulence factors via quorum sensing (QS). Some bacteria produce enzymes to inactivate the AHL signals of pathogenic bacteria via a phenomenon known as quorum quenching. One hundred bacterial isolates from potato tubers were screened for AHL-degrading activity using biosensor strain Chromobacterium violaceum CV026. Of these isolates, 20 were able to inactivate AHLs from the pathogenic bacteria in vitro. Of the 20 isolates, 6 attenuated tissue maceration of potato tubers by Pc. Suppression of tuber soft rot was observed even when these isolates were applied 24 h after the pathogen was introduced. Their colonization in tubers was approximately 103-104 cfu/g tuber, 7 days after inoculation. These isolates were identified as Bacillus sp., Variovorax sp., Variovorax paradoxus and Agrobacterium tumefaciens. Four of these isolates showed putative AHL-lactonase activity and provided the most significant protection against Pc. Therefore, AHL-degrading endophytic bacteria can be utilized as a novel biocontrol agent of potato tuber soft rot in Vietnam.
AB - Pectobacterium carotovorum (Pc) causing potato tuber soft rot uses N-acyl-L-homoserine lactones (AHLs) to control the production of virulence factors via quorum sensing (QS). Some bacteria produce enzymes to inactivate the AHL signals of pathogenic bacteria via a phenomenon known as quorum quenching. One hundred bacterial isolates from potato tubers were screened for AHL-degrading activity using biosensor strain Chromobacterium violaceum CV026. Of these isolates, 20 were able to inactivate AHLs from the pathogenic bacteria in vitro. Of the 20 isolates, 6 attenuated tissue maceration of potato tubers by Pc. Suppression of tuber soft rot was observed even when these isolates were applied 24 h after the pathogen was introduced. Their colonization in tubers was approximately 103-104 cfu/g tuber, 7 days after inoculation. These isolates were identified as Bacillus sp., Variovorax sp., Variovorax paradoxus and Agrobacterium tumefaciens. Four of these isolates showed putative AHL-lactonase activity and provided the most significant protection against Pc. Therefore, AHL-degrading endophytic bacteria can be utilized as a novel biocontrol agent of potato tuber soft rot in Vietnam.
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U2 - 10.18520/cs/v115/i10/1921-1927
DO - 10.18520/cs/v115/i10/1921-1927
M3 - Article
VL - 115
SP - 1921
EP - 1927
JO - Current Science
JF - Current Science
SN - 0011-3891
IS - 10
ER -