Biological immunoassay with high Tc superconducting quantum interference device (SQUID) magnetometer

Keiji Enpuku, Tadashi Minutant

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A high Tc superconducting quantum interference device (SQUID) magnetometer system is developed for the application to biological immunoassay. In this application, magnetic nanoparticles are used as magnetic markers to perform immunoassay, i.e., to detect binding reaction between an antigen and its antibody. The antibody is labeled with γ-Fe2O3 nanoparticles, and the binding reaction can be magnetically detected by measuring the magnetic field from the nanoparticles. Design and set up of the system is described, and the sensitivity of the system is studied in terms of detectable number of the magnetic markers. At present, we can detect 4×106 markers when the diameter of the marker is 50 nm. Total weight of the magnetic nanoparticles becomes 520 pg in this case. An experiment is also conducted to measure antigen-antibody reaction with the present system. It is shown that the sensitivity of the present system is 10 times better than that of the conventional method using an optical marker. A one order of magnitude improvement of sensitivity will be realized by the sophistication of the present system.

Original languageEnglish
Pages (from-to)43-48
Number of pages6
JournalIEICE Transactions on Electronics
VolumeE84-C
Issue number1
Publication statusPublished - Jan 1 2001

Fingerprint

SQUIDs
Magnetometers
Nanoparticles
Antibodies
Antigen-antibody reactions
Antigens
Magnetic fields
Experiments

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Electrical and Electronic Engineering

Cite this

Biological immunoassay with high Tc superconducting quantum interference device (SQUID) magnetometer. / Enpuku, Keiji; Minutant, Tadashi.

In: IEICE Transactions on Electronics, Vol. E84-C, No. 1, 01.01.2001, p. 43-48.

Research output: Contribution to journalArticle

@article{c700266e48c94da398bc16c9df119582,
title = "Biological immunoassay with high Tc superconducting quantum interference device (SQUID) magnetometer",
abstract = "A high Tc superconducting quantum interference device (SQUID) magnetometer system is developed for the application to biological immunoassay. In this application, magnetic nanoparticles are used as magnetic markers to perform immunoassay, i.e., to detect binding reaction between an antigen and its antibody. The antibody is labeled with γ-Fe2O3 nanoparticles, and the binding reaction can be magnetically detected by measuring the magnetic field from the nanoparticles. Design and set up of the system is described, and the sensitivity of the system is studied in terms of detectable number of the magnetic markers. At present, we can detect 4×106 markers when the diameter of the marker is 50 nm. Total weight of the magnetic nanoparticles becomes 520 pg in this case. An experiment is also conducted to measure antigen-antibody reaction with the present system. It is shown that the sensitivity of the present system is 10 times better than that of the conventional method using an optical marker. A one order of magnitude improvement of sensitivity will be realized by the sophistication of the present system.",
author = "Keiji Enpuku and Tadashi Minutant",
year = "2001",
month = "1",
day = "1",
language = "English",
volume = "E84-C",
pages = "43--48",
journal = "IEICE Transactions on Electronics",
issn = "0916-8524",
publisher = "The Institute of Electronics, Information and Communication Engineers (IEICE)",
number = "1",

}

TY - JOUR

T1 - Biological immunoassay with high Tc superconducting quantum interference device (SQUID) magnetometer

AU - Enpuku, Keiji

AU - Minutant, Tadashi

PY - 2001/1/1

Y1 - 2001/1/1

N2 - A high Tc superconducting quantum interference device (SQUID) magnetometer system is developed for the application to biological immunoassay. In this application, magnetic nanoparticles are used as magnetic markers to perform immunoassay, i.e., to detect binding reaction between an antigen and its antibody. The antibody is labeled with γ-Fe2O3 nanoparticles, and the binding reaction can be magnetically detected by measuring the magnetic field from the nanoparticles. Design and set up of the system is described, and the sensitivity of the system is studied in terms of detectable number of the magnetic markers. At present, we can detect 4×106 markers when the diameter of the marker is 50 nm. Total weight of the magnetic nanoparticles becomes 520 pg in this case. An experiment is also conducted to measure antigen-antibody reaction with the present system. It is shown that the sensitivity of the present system is 10 times better than that of the conventional method using an optical marker. A one order of magnitude improvement of sensitivity will be realized by the sophistication of the present system.

AB - A high Tc superconducting quantum interference device (SQUID) magnetometer system is developed for the application to biological immunoassay. In this application, magnetic nanoparticles are used as magnetic markers to perform immunoassay, i.e., to detect binding reaction between an antigen and its antibody. The antibody is labeled with γ-Fe2O3 nanoparticles, and the binding reaction can be magnetically detected by measuring the magnetic field from the nanoparticles. Design and set up of the system is described, and the sensitivity of the system is studied in terms of detectable number of the magnetic markers. At present, we can detect 4×106 markers when the diameter of the marker is 50 nm. Total weight of the magnetic nanoparticles becomes 520 pg in this case. An experiment is also conducted to measure antigen-antibody reaction with the present system. It is shown that the sensitivity of the present system is 10 times better than that of the conventional method using an optical marker. A one order of magnitude improvement of sensitivity will be realized by the sophistication of the present system.

UR - http://www.scopus.com/inward/record.url?scp=0035117750&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035117750&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0035117750

VL - E84-C

SP - 43

EP - 48

JO - IEICE Transactions on Electronics

JF - IEICE Transactions on Electronics

SN - 0916-8524

IS - 1

ER -