Abstract
We have developed a magnetic immunoassay system utilizing a magnetic marker and HTS SQUID. In this system, the magnetic marker was used to detect the biological material called antigen. The magnetic marker was designed so as to generate a remanence, and the remanence field of the markers that bound to the antigens was measured with the SQUTD. The measurement was performed In a solution that contained both the bound and free (or unbound) markers, i.e., without using the so-called Bound/Free (BF) separation process. The Brownian rotation of the free markers in the solution was used to distinguish the bound markers from the free ones. Using the system, we conducted the detection of the biological material called human IgE. Good relationship was obtained between the detected signal and the weight of IgE. Minimum detectable weight of IgE was 2.4 pg (or 15 atto-mol). This sensitivity was limited by the background signal from the free markers.
| Original language | English |
|---|---|
| Pages (from-to) | 816-819 |
| Number of pages | 4 |
| Journal | IEEE Transactions on Applied Superconductivity |
| Volume | 17 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jun 1 2007 |
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Electrical and Electronic Engineering
Cite this
Biological immunoassays without bound/free separation utilizing magnetic marker and HTS SQUID. / Enpuku, Keiji; Soejima, K.; Nishimoto, T.; Matsuda, T.; Tokumitsu, H.; Tanaka, T.; Yoshinaga, K.; Kuma, H.; Hamasaki, N.
In: IEEE Transactions on Applied Superconductivity, Vol. 17, No. 2, 01.06.2007, p. 816-819.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Biological immunoassays without bound/free separation utilizing magnetic marker and HTS SQUID
AU - Enpuku, Keiji
AU - Soejima, K.
AU - Nishimoto, T.
AU - Matsuda, T.
AU - Tokumitsu, H.
AU - Tanaka, T.
AU - Yoshinaga, K.
AU - Kuma, H.
AU - Hamasaki, N.
PY - 2007/6/1
Y1 - 2007/6/1
N2 - We have developed a magnetic immunoassay system utilizing a magnetic marker and HTS SQUID. In this system, the magnetic marker was used to detect the biological material called antigen. The magnetic marker was designed so as to generate a remanence, and the remanence field of the markers that bound to the antigens was measured with the SQUTD. The measurement was performed In a solution that contained both the bound and free (or unbound) markers, i.e., without using the so-called Bound/Free (BF) separation process. The Brownian rotation of the free markers in the solution was used to distinguish the bound markers from the free ones. Using the system, we conducted the detection of the biological material called human IgE. Good relationship was obtained between the detected signal and the weight of IgE. Minimum detectable weight of IgE was 2.4 pg (or 15 atto-mol). This sensitivity was limited by the background signal from the free markers.
AB - We have developed a magnetic immunoassay system utilizing a magnetic marker and HTS SQUID. In this system, the magnetic marker was used to detect the biological material called antigen. The magnetic marker was designed so as to generate a remanence, and the remanence field of the markers that bound to the antigens was measured with the SQUTD. The measurement was performed In a solution that contained both the bound and free (or unbound) markers, i.e., without using the so-called Bound/Free (BF) separation process. The Brownian rotation of the free markers in the solution was used to distinguish the bound markers from the free ones. Using the system, we conducted the detection of the biological material called human IgE. Good relationship was obtained between the detected signal and the weight of IgE. Minimum detectable weight of IgE was 2.4 pg (or 15 atto-mol). This sensitivity was limited by the background signal from the free markers.
UR - http://www.scopus.com/inward/record.url?scp=34547473130&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34547473130&partnerID=8YFLogxK
U2 - 10.1109/TASC.2007.897699
DO - 10.1109/TASC.2007.897699
M3 - Article
VL - 17
SP - 816
EP - 819
JO - IEEE Transactions on Applied Superconductivity
JF - IEEE Transactions on Applied Superconductivity
SN - 1051-8223
IS - 2
ER -