Biological immunoassays without bound/free separation utilizing magnetic marker and HTS SQUID

Keiji Enpuku, K. Soejima, T. Nishimoto, T. Matsuda, H. Tokumitsu, T. Tanaka, K. Yoshinaga, H. Kuma, N. Hamasaki

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We have developed a magnetic immunoassay system utilizing a magnetic marker and HTS SQUID. In this system, the magnetic marker was used to detect the biological material called antigen. The magnetic marker was designed so as to generate a remanence, and the remanence field of the markers that bound to the antigens was measured with the SQUTD. The measurement was performed In a solution that contained both the bound and free (or unbound) markers, i.e., without using the so-called Bound/Free (BF) separation process. The Brownian rotation of the free markers in the solution was used to distinguish the bound markers from the free ones. Using the system, we conducted the detection of the biological material called human IgE. Good relationship was obtained between the detected signal and the weight of IgE. Minimum detectable weight of IgE was 2.4 pg (or 15 atto-mol). This sensitivity was limited by the background signal from the free markers.

Original languageEnglish
Pages (from-to)816-819
Number of pages4
JournalIEEE Transactions on Applied Superconductivity
Volume17
Issue number2
DOIs
Publication statusPublished - Jun 1 2007

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Magnetic separation
immunoassay
Remanence
SQUIDs
Antigens
markers
Biological materials
Immunoglobulin E
antigens
remanence
sensitivity

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Enpuku, K., Soejima, K., Nishimoto, T., Matsuda, T., Tokumitsu, H., Tanaka, T., ... Hamasaki, N. (2007). Biological immunoassays without bound/free separation utilizing magnetic marker and HTS SQUID. IEEE Transactions on Applied Superconductivity, 17(2), 816-819. https://doi.org/10.1109/TASC.2007.897699

Biological immunoassays without bound/free separation utilizing magnetic marker and HTS SQUID. / Enpuku, Keiji; Soejima, K.; Nishimoto, T.; Matsuda, T.; Tokumitsu, H.; Tanaka, T.; Yoshinaga, K.; Kuma, H.; Hamasaki, N.

In: IEEE Transactions on Applied Superconductivity, Vol. 17, No. 2, 01.06.2007, p. 816-819.

Research output: Contribution to journalArticle

Enpuku, K, Soejima, K, Nishimoto, T, Matsuda, T, Tokumitsu, H, Tanaka, T, Yoshinaga, K, Kuma, H & Hamasaki, N 2007, 'Biological immunoassays without bound/free separation utilizing magnetic marker and HTS SQUID', IEEE Transactions on Applied Superconductivity, vol. 17, no. 2, pp. 816-819. https://doi.org/10.1109/TASC.2007.897699
Enpuku, Keiji ; Soejima, K. ; Nishimoto, T. ; Matsuda, T. ; Tokumitsu, H. ; Tanaka, T. ; Yoshinaga, K. ; Kuma, H. ; Hamasaki, N. / Biological immunoassays without bound/free separation utilizing magnetic marker and HTS SQUID. In: IEEE Transactions on Applied Superconductivity. 2007 ; Vol. 17, No. 2. pp. 816-819.
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