Biological immunoassays without bound/free separation utilizing magnetic marker and HTS SQUID

K. Enpuku; K. Soejima; T. Nishimoto; T. Matsuda; H. Tokumitsu; T. Tanaka; K. Yoshinaga; H. Kuma; N. Hamasaki

doi:10.1109/TASC.2007.897699

Biological immunoassays without bound/free separation utilizing magnetic marker and HTS SQUID

K. Enpuku, K. Soejima, T. Nishimoto, T. Matsuda, H. Tokumitsu, T. Tanaka, K. Yoshinaga, H. Kuma, N. Hamasaki

Superconductive Systems Engineering Laboratory

Research output: Contribution to journal › Article

22 Citations (Scopus)

Abstract

We have developed a magnetic immunoassay system utilizing a magnetic marker and HTS SQUID. In this system, the magnetic marker was used to detect the biological material called antigen. The magnetic marker was designed so as to generate a remanence, and the remanence field of the markers that bound to the antigens was measured with the SQUTD. The measurement was performed In a solution that contained both the bound and free (or unbound) markers, i.e., without using the so-called Bound/Free (BF) separation process. The Brownian rotation of the free markers in the solution was used to distinguish the bound markers from the free ones. Using the system, we conducted the detection of the biological material called human IgE. Good relationship was obtained between the detected signal and the weight of IgE. Minimum detectable weight of IgE was 2.4 pg (or 15 atto-mol). This sensitivity was limited by the background signal from the free markers.

Original language	English
Pages (from-to)	816-819
Number of pages	4
Journal	IEEE Transactions on Applied Superconductivity
Volume	17
Issue number	2
DOIs	https://doi.org/10.1109/TASC.2007.897699
Publication status	Published - Jun 1 2007

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Electrical and Electronic Engineering

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Enpuku, K., Soejima, K., Nishimoto, T., Matsuda, T., Tokumitsu, H., Tanaka, T., Yoshinaga, K., Kuma, H., & Hamasaki, N. (2007). Biological immunoassays without bound/free separation utilizing magnetic marker and HTS SQUID. IEEE Transactions on Applied Superconductivity, 17(2), 816-819. https://doi.org/10.1109/TASC.2007.897699

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abstract = "We have developed a magnetic immunoassay system utilizing a magnetic marker and HTS SQUID. In this system, the magnetic marker was used to detect the biological material called antigen. The magnetic marker was designed so as to generate a remanence, and the remanence field of the markers that bound to the antigens was measured with the SQUTD. The measurement was performed In a solution that contained both the bound and free (or unbound) markers, i.e., without using the so-called Bound/Free (BF) separation process. The Brownian rotation of the free markers in the solution was used to distinguish the bound markers from the free ones. Using the system, we conducted the detection of the biological material called human IgE. Good relationship was obtained between the detected signal and the weight of IgE. Minimum detectable weight of IgE was 2.4 pg (or 15 atto-mol). This sensitivity was limited by the background signal from the free markers.",

author = "K. Enpuku and K. Soejima and T. Nishimoto and T. Matsuda and H. Tokumitsu and T. Tanaka and K. Yoshinaga and H. Kuma and N. Hamasaki",

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AU - Enpuku, K.

AU - Soejima, K.

AU - Nishimoto, T.

AU - Matsuda, T.

AU - Tokumitsu, H.

AU - Tanaka, T.

AU - Yoshinaga, K.

AU - Kuma, H.

AU - Hamasaki, N.

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