Biological nano-mineralization of Ce phosphate by Saccharomyces cerevisiae

Mingyu Jiang, Toshihiko Ohnuki, Naofumi Kozai, Kazuya Tanaka, Yoshinori Suzuki, Fuminori Sakamoto, Eigo Kamiishi, Satoshi Utsunomiya

Research output: Contribution to journalArticle

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Abstract

In order to understand the nanoscale mineralization process of REE phosphate on microorganism surfaces, we have investigated the mechanism underlying Ce sequestration by yeast Saccharomyces cerevisiae after exposure to Ce(III) solution for 4-120. h at pH 3, 4, or 5. A variety of analytical techniques have been employed, including field-emission scanning electron microscopy equipped with energy-dispersive X-ray spectroscopy (FESEM-EDS), transmission electron microscopy (TEM), inductively coupled plasma atomic emission spectrometry (ICP-AES), and synchrotron-based X-ray absorption fine structure (XAFS).Cerium concentration in solutions decreases as a function of exposure time and more rapidly at pH 5 than at pH 3 or 4. Analyses of the yeast cells by FESEM, TEM, and XAFS show that needle-shaped Ce(III) phosphate nanocrystallites with a monazite structure formed on the yeast cells by exposure to Ce(III) for 42. h, even though the initial solutions did not contain any P species. The Ce(III) phosphate nanocrystals grew from about 50. nm to hundreds of nanometers when pH increased from 3 to 5. Lower pH resulted in higher P concentration in the solution after the yeast cells were inoculated, indicating the release of P from the yeast cells. These results suggest that the sorbed Ce on the cell surfaces reacted with P released from inside the yeast cell, resulting in the formation of Ce(III) phosphate nanocrystallites. This post-sorption nanocrystallization on the microbial cell surface should play a key role in constraining the long-term migration of REEs and trivalent actinides in geological repositories.

Original languageEnglish
Pages (from-to)61-69
Number of pages9
JournalChemical Geology
Volume277
Issue number1-2
DOIs
Publication statusPublished - Oct 1 2010

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Yeast
yeast
Phosphates
phosphate
mineralization
Cells
Nanocrystallites
X ray absorption
transmission electron microscopy
rare earth element
Actinoid Series Elements
Geological repositories
actinide
cerium
Nanocrystallization
Transmission electron microscopy
Cerium
monazite
repository
Inductively coupled plasma

All Science Journal Classification (ASJC) codes

  • Geology
  • Geochemistry and Petrology

Cite this

Biological nano-mineralization of Ce phosphate by Saccharomyces cerevisiae. / Jiang, Mingyu; Ohnuki, Toshihiko; Kozai, Naofumi; Tanaka, Kazuya; Suzuki, Yoshinori; Sakamoto, Fuminori; Kamiishi, Eigo; Utsunomiya, Satoshi.

In: Chemical Geology, Vol. 277, No. 1-2, 01.10.2010, p. 61-69.

Research output: Contribution to journalArticle

Jiang, M, Ohnuki, T, Kozai, N, Tanaka, K, Suzuki, Y, Sakamoto, F, Kamiishi, E & Utsunomiya, S 2010, 'Biological nano-mineralization of Ce phosphate by Saccharomyces cerevisiae', Chemical Geology, vol. 277, no. 1-2, pp. 61-69. https://doi.org/10.1016/j.chemgeo.2010.07.010
Jiang M, Ohnuki T, Kozai N, Tanaka K, Suzuki Y, Sakamoto F et al. Biological nano-mineralization of Ce phosphate by Saccharomyces cerevisiae. Chemical Geology. 2010 Oct 1;277(1-2):61-69. https://doi.org/10.1016/j.chemgeo.2010.07.010
Jiang, Mingyu ; Ohnuki, Toshihiko ; Kozai, Naofumi ; Tanaka, Kazuya ; Suzuki, Yoshinori ; Sakamoto, Fuminori ; Kamiishi, Eigo ; Utsunomiya, Satoshi. / Biological nano-mineralization of Ce phosphate by Saccharomyces cerevisiae. In: Chemical Geology. 2010 ; Vol. 277, No. 1-2. pp. 61-69.
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