Biological selection of peptides for poly(L-lactide) substrates

Hisao Matsuno, Jun Sekine, Hirofumi Yajima, Takeshi Serizawa

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Short peptides that recognize the a form of poly(L-lactide) (PLLA) crystalline films were identified from a phagedisplayed peptide library. An enzyme-linked immunosorbent assay (ELISA) revealed that the apparent binding constants of the phage clones for the a form of PLLA were greater than those of the unselected phage library. The specificity index for the a form of PLLA referred to a structurally similar atactic poly(methyl methacrylate) (at-PMMA), supporting the a form of PLLA specific binding of the selected phage. Amino acid residues with proton-donor lateral groups and hydrophobic alkyl groups were relatively enriched in a sequence of heptapeptides on the specific phage clones, thereby suggesting the presence of hydrogen bonding as well as hydrophobic interactions between the a form of PLLA and the peptides. Surface plasmon resonance (SPR) analysis revealed that the binding constant of the freed c22 heptapeptide (Gln-Leu-Met-His-Asp-Tyr-Arg) for the a form of PLLA was greater than those for reference at-PMMA, amorphous PLLA, and the β form of PLLA. It was found that c22 peptide can recognize slight differences in PLLA polymorphs such as a crystalline state and an arrangement of PLLA functional groups.

Original languageEnglish
Pages (from-to)6399-6403
Number of pages5
JournalLangmuir
Volume24
Issue number13
DOIs
Publication statusPublished - Jul 1 2008

Fingerprint

Bacteriophages
Peptides
peptides
Substrates
Polymethyl methacrylates
polymethyl methacrylate
Crystalline materials
Surface plasmon resonance
Polymorphism
surface plasmon resonance
Functional groups
amino acids
Amino acids
enzymes
Polymethyl Methacrylate
Assays
Hydrogen bonds
Protons
Enzymes
protons

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Biological selection of peptides for poly(L-lactide) substrates. / Matsuno, Hisao; Sekine, Jun; Yajima, Hirofumi; Serizawa, Takeshi.

In: Langmuir, Vol. 24, No. 13, 01.07.2008, p. 6399-6403.

Research output: Contribution to journalArticle

Matsuno, H, Sekine, J, Yajima, H & Serizawa, T 2008, 'Biological selection of peptides for poly(L-lactide) substrates', Langmuir, vol. 24, no. 13, pp. 6399-6403. https://doi.org/10.1021/la8008442
Matsuno, Hisao ; Sekine, Jun ; Yajima, Hirofumi ; Serizawa, Takeshi. / Biological selection of peptides for poly(L-lactide) substrates. In: Langmuir. 2008 ; Vol. 24, No. 13. pp. 6399-6403.
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