Biologically active human bone morphogenetic protein 4 fused to collagen-binding domain produced in silkworm-baculovirus expression system

Saki Imai, Zhiqing Li, Kazuhiro Iiyama, Yoshitaka Miyagawa, Masashi Toyoda, Akihiro Umezawa, Hiroaki Mon, Takahiro Kusakabe, Kaito Yoshimura, Jae Man Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Human bone morphogenetic protein 4 (BMP4) fused to the collagen-binding domain (CBD) of fbronectin was constructed and expressed in a silkworm-baculovirus expression system. When recombinant BmNPV was injected into the silkworm larvae and harvested after approximately 4 days, 0.22 mg/ml (0.1 mg/larva) of recombinant CBD-BMP4 was secreted into the silkworm haemolymph. Interestingly, cultured silkworm cells infected with the same recombinant virus could not secrete the recombinant CBD-BMP4 into culture media. The purifed rCBD-BMP4 showed Smad- and MAPK-stimulating activities in human UET-13 cells.

Original languageEnglish
Pages (from-to)39-44
Number of pages6
JournalJournal of Insect Biotechnology and Sericology
Volume82
Issue number2
DOIs
Publication statusPublished - Dec 12 2013

Fingerprint

bone morphogenetic proteins
Bombyx
Baculoviridae
Bone Morphogenetic Protein 4
silkworms
Collagen
collagen
Bone
Proteins
Larva
Bombyx mori nucleopolyhedrovirus
Hemolymph
Viruses
Human Activities
hemolymph
Culture Media
insect larvae
Cultured Cells
culture media
cells

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Business, Management and Accounting(all)
  • Agricultural and Biological Sciences(all)
  • Insect Science
  • Industrial and Manufacturing Engineering

Cite this

Biologically active human bone morphogenetic protein 4 fused to collagen-binding domain produced in silkworm-baculovirus expression system. / Imai, Saki; Li, Zhiqing; Iiyama, Kazuhiro; Miyagawa, Yoshitaka; Toyoda, Masashi; Umezawa, Akihiro; Mon, Hiroaki; Kusakabe, Takahiro; Yoshimura, Kaito; Lee, Jae Man.

In: Journal of Insect Biotechnology and Sericology, Vol. 82, No. 2, 12.12.2013, p. 39-44.

Research output: Contribution to journalArticle

Imai, Saki ; Li, Zhiqing ; Iiyama, Kazuhiro ; Miyagawa, Yoshitaka ; Toyoda, Masashi ; Umezawa, Akihiro ; Mon, Hiroaki ; Kusakabe, Takahiro ; Yoshimura, Kaito ; Lee, Jae Man. / Biologically active human bone morphogenetic protein 4 fused to collagen-binding domain produced in silkworm-baculovirus expression system. In: Journal of Insect Biotechnology and Sericology. 2013 ; Vol. 82, No. 2. pp. 39-44.
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abstract = "Human bone morphogenetic protein 4 (BMP4) fused to the collagen-binding domain (CBD) of fbronectin was constructed and expressed in a silkworm-baculovirus expression system. When recombinant BmNPV was injected into the silkworm larvae and harvested after approximately 4 days, 0.22 mg/ml (0.1 mg/larva) of recombinant CBD-BMP4 was secreted into the silkworm haemolymph. Interestingly, cultured silkworm cells infected with the same recombinant virus could not secrete the recombinant CBD-BMP4 into culture media. The purifed rCBD-BMP4 showed Smad- and MAPK-stimulating activities in human UET-13 cells.",
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AU - Miyagawa, Yoshitaka

AU - Toyoda, Masashi

AU - Umezawa, Akihiro

AU - Mon, Hiroaki

AU - Kusakabe, Takahiro

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