TY - JOUR
T1 - Bioluminescent system for dynamic imaging of cell and animal behavior
AU - Hara-Miyauchi, Chikako
AU - Tsuji, Osahiko
AU - Hanyu, Aki
AU - Okada, Seiji
AU - Yasuda, Akimasa
AU - Fukano, Takashi
AU - Akazawa, Chihiro
AU - Nakamura, Masaya
AU - Imamura, Takeshi
AU - Matsuzaki, Yumi
AU - Okano, Hirotaka James
AU - Miyawaki, Atsushi
AU - Okano, Hideyuki
N1 - Funding Information:
We thank K. Hara and H. Mizuno for helpful discussions, S. Nori for technical support, and T. Harada for animal care. Requests for ffLuc vector should be addressed to Atsushi Miyawaki, and requests for ffLuc mice should be addressed to Hideyuki Okano. Atsushi Miyawaki and Hideyuki Okano contributed equally to this paper. This work was supported by Grants from The Japanese Ministry of Education, Culture Sports, Science, and Technology (MEXT) to Atsushi Miyawaki and Hideyuki Okano, and from “ Funding Program for World-leading Innovative R&D on Science and Technology ” to Hideyuki Okano.
PY - 2012/3/9
Y1 - 2012/3/9
N2 - The current utility of bioluminescence imaging is constrained by a low photon yield that limits temporal sensitivity. Here, we describe an imaging method that uses a chemiluminescent/fluorescent protein, . ffLuc-cp156, which consists of a yellow variant of . Aequorea GFP and firefly luciferase. We report an improvement in photon yield by over three orders of magnitude over current bioluminescent systems. We imaged cellular movement at high resolution including neuronal growth cones and microglial cell protrusions. Transgenic . ffLuc-cp156 mice enabled video-rate bioluminescence imaging of freely moving animals, which may provide a reliable assay for drug distribution in behaving animals for pre-clinical studies.
AB - The current utility of bioluminescence imaging is constrained by a low photon yield that limits temporal sensitivity. Here, we describe an imaging method that uses a chemiluminescent/fluorescent protein, . ffLuc-cp156, which consists of a yellow variant of . Aequorea GFP and firefly luciferase. We report an improvement in photon yield by over three orders of magnitude over current bioluminescent systems. We imaged cellular movement at high resolution including neuronal growth cones and microglial cell protrusions. Transgenic . ffLuc-cp156 mice enabled video-rate bioluminescence imaging of freely moving animals, which may provide a reliable assay for drug distribution in behaving animals for pre-clinical studies.
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U2 - 10.1016/j.bbrc.2012.01.141
DO - 10.1016/j.bbrc.2012.01.141
M3 - Article
C2 - 22333570
AN - SCOPUS:84857993014
SN - 0006-291X
VL - 419
SP - 188
EP - 193
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -