Biomimetic diamond-like carbon coated titanium inhibits RANKL-dependent osteoclast differentiation in vitro

Yuichi Mine, Tatsuyuki Nakatani, Keishi Okamoto, Seicho Makihira, Satoshi Iwata, Hiroki Nikawa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

To examine the effects of titanium (Ti) coated with biomimetic diamond-like carbon (DLC) on osteoclast differentiation and bacterial biofilm formation, RAW264.7 cells and Porphyromonas gingivalis (P. g) were grown on Ti disks without DLC (control), or with DLC (DLC-treated). Real-time quantitative reverse transcriptase-polymerase chain reaction analysis showed that TRAP and cathepsin K mRNA levels in RAW264.7 cultured on DLC-treated Ti disks with soluble receptor activators for NF-κB ligand (sRANKL) were significantly lower than those of control Ti disks. Similarly, DLC-treated Ti decreased the expression of NFATcl protein in sRANKL-stimulated RAW264.7 cells. Futher, reduced P. g biofllm formation was observed on DLC-treated disks as compared with the biofilm on control Ti disks. These results, taken together, suggested that DLC altered osteoclast differentiation and biofllm formation of P.g, which could promise long-term success of implant therapy.

Original languageEnglish
Pages (from-to)523-528
Number of pages6
JournalJournal of Photopolymer Science and Technology
Volume25
Issue number4
DOIs
Publication statusPublished - Oct 30 2012

Fingerprint

Diamond
Biomimetics
Titanium
Diamonds
Carbon
Biofilms
Cathepsin K
Osteoclasts
Polymerase chain reaction
RNA-Directed DNA Polymerase
Ligands
Proteins
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics
  • Organic Chemistry
  • Materials Chemistry

Cite this

Biomimetic diamond-like carbon coated titanium inhibits RANKL-dependent osteoclast differentiation in vitro. / Mine, Yuichi; Nakatani, Tatsuyuki; Okamoto, Keishi; Makihira, Seicho; Iwata, Satoshi; Nikawa, Hiroki.

In: Journal of Photopolymer Science and Technology, Vol. 25, No. 4, 30.10.2012, p. 523-528.

Research output: Contribution to journalArticle

Mine, Yuichi ; Nakatani, Tatsuyuki ; Okamoto, Keishi ; Makihira, Seicho ; Iwata, Satoshi ; Nikawa, Hiroki. / Biomimetic diamond-like carbon coated titanium inhibits RANKL-dependent osteoclast differentiation in vitro. In: Journal of Photopolymer Science and Technology. 2012 ; Vol. 25, No. 4. pp. 523-528.
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