Biomimetic self-templating optical structures fabricated by genetically engineered M13 bacteriophage

Won Geun Kim, Hyerin Song, Chuntae Kim, Jong Sik Moon, Kyujung Kim, Seung Wuk Lee, Jin Woo Oh

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


Here, we describe a highly sensitive and selective surface plasmon resonance sensor system by utilizing self-assembly of genetically engineered M13 bacteriophage. About 2700 copies of genetically expressed peptide copies give superior selectivity and sensitivity to M13 phage-based SPR sensor. Furthermore, the sensitivity of the M13 phage-based SPR sensor was enhanced due to the aligning of receptor matrix in specific direction. Incorporation of specific binding peptide (His Pro Gln: HPQ) gives M13 bacteriophage high selectivity for the streptavidin. Our M13 phage-based SPR sensor takes advantage of simplicity of self-assembly compared with relatively complex photolithography techniques or chemical conjugations. Additionally, designed structure which is composed of functionalized M13 bacteriophage can simultaneously improve the sensitivity and selectivity of SPR sensor evidently. By taking advantages of the genetic engineering and self-assembly, we propose the simple method for fabricating novel M13 phage-based SPR sensor system which has a high sensitivity and high selectivity.

Original languageEnglish
Pages (from-to)853-859
Number of pages7
JournalBiosensors and Bioelectronics
Publication statusPublished - Nov 15 2016
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry


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