Biomolecular motor modulates mechanical property of microtubule

Arif Md Rashedul Kabir, Daisuke Inoue, Yoshimi Hamano, Hiroyuki Mayama, Kazuki Sada, Akira Kakugo

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The microtubule (MT) is the stiffest cytoskeletal filamentous protein that takes part in a wide range of cellular activities where its mechanical property plays a crucially significant role. How a single biological entity plays multiple roles in cell has been a mystery for long time. Over the recent years, it has been known that modulation of the mechanical property of MT by different cellular agents is the key to performing manifold in vivo activities by MT. Studying the mechanical property of MT thus has been a prerequisite in understanding how MT plays such diversified in vivo roles. However, the anisotropic structure of MT has been an impediment in obtaining a precise description of the mechanical property of MT along its longitudinal and lateral directions that requires employment of distinct experimental approach and has not been demonstrated yet. In this work, we have developed an experimental system that enabled us to investigate the effect of tensile stress on MT. By using our newly developed system, (1) we have determined the Youngs modulus of MT considering its deformation under applied tensile stress and (2) a new role of MT associated motor protein kinesin in modulating the mechanical property of MT was revealed for the first time. Decrease in Youngs modulus of MT with the increase in interaction with kinesin suggests that kinesin has a softening effect on MT and thereby can modulate the rigidity of MT. This work will be an aid in understanding the modulation of mechanical property of MTs by MT associated proteins and might also help obtain a clear insight of the endurance and mechanical instability of MTs under applied stress.

Original languageEnglish
Pages (from-to)1797-1805
Number of pages9
JournalBiomacromolecules
Volume15
Issue number5
DOIs
Publication statusPublished - May 12 2014
Externally publishedYes

Fingerprint

Kinesin
Mechanical properties
Proteins
Tensile stress
Elastic moduli
Modulation
Microtubule-Associated Proteins
Rigidity
Durability

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Kabir, A. M. R., Inoue, D., Hamano, Y., Mayama, H., Sada, K., & Kakugo, A. (2014). Biomolecular motor modulates mechanical property of microtubule. Biomacromolecules, 15(5), 1797-1805. https://doi.org/10.1021/bm5001789

Biomolecular motor modulates mechanical property of microtubule. / Kabir, Arif Md Rashedul; Inoue, Daisuke; Hamano, Yoshimi; Mayama, Hiroyuki; Sada, Kazuki; Kakugo, Akira.

In: Biomacromolecules, Vol. 15, No. 5, 12.05.2014, p. 1797-1805.

Research output: Contribution to journalArticle

Kabir, AMR, Inoue, D, Hamano, Y, Mayama, H, Sada, K & Kakugo, A 2014, 'Biomolecular motor modulates mechanical property of microtubule', Biomacromolecules, vol. 15, no. 5, pp. 1797-1805. https://doi.org/10.1021/bm5001789
Kabir AMR, Inoue D, Hamano Y, Mayama H, Sada K, Kakugo A. Biomolecular motor modulates mechanical property of microtubule. Biomacromolecules. 2014 May 12;15(5):1797-1805. https://doi.org/10.1021/bm5001789
Kabir, Arif Md Rashedul ; Inoue, Daisuke ; Hamano, Yoshimi ; Mayama, Hiroyuki ; Sada, Kazuki ; Kakugo, Akira. / Biomolecular motor modulates mechanical property of microtubule. In: Biomacromolecules. 2014 ; Vol. 15, No. 5. pp. 1797-1805.
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