Biomolecular recognition on nanowire surfaces modified by the self-assembled monolayer

Taisuke Shimada, Takao Yasui, Asami Yokoyama, Tatsuro Goda, Mitsuo Hara, Takeshi Yanagida, Noritada Kaji, Masaki Kanai, Kazuki Nagashima, Yuji Miyahara, Tomoji Kawai, Yoshinobu Baba

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Molecular recognition is one of the key factors in designing biosensors due to which nanowires functionalized with molecular recognition have attracted a lot of attention as promising candidates for nanostructures embedded in biosensors. However, the difficulty in real-world applications with analytical specificity is that molecular recognition on nanowires mainly depends on antibody modification with multistep modification procedures. Furthermore, the antibody modification suffers from nonspecific adsorption of undesired proteins in body fluid on the nanowires, which causes false responses and lowers sensitivity. Herein, we propose biomolecular recognition using surface-modified nanowires via thiolated 2-methacryloxyethyl phosphorylcholine (MPC-SH). MPC-SH enables self-assembled monolayer (SAM) modification, which contributes to the reduction of nonspecific adsorption of biomolecules onto the nanowires, and the specific capture of a target protein is attained in the presence of calcium ions. Our concept demonstrates the recognition of the biomarker protein on nanowire surfaces modified by MPC-SH SAM with a single step modification procedure.

Original languageEnglish
Pages (from-to)3225-3229
Number of pages5
JournalLab on a Chip
Volume18
Issue number21
DOIs
Publication statusPublished - Nov 7 2018

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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