Biomolecular recognition on nanowire surfaces modified by the self-assembled monolayer

Taisuke Shimada, Takao Yasui, Asami Yokoyama, Tatsuro Goda, Mitsuo Hara, Takeshi Yanagida, Noritada Kaji, Masaki Kanai, Kazuki Nagashima, Yuji Miyahara, Tomoji Kawai, Yoshinobu Baba

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Molecular recognition is one of the key factors in designing biosensors due to which nanowires functionalized with molecular recognition have attracted a lot of attention as promising candidates for nanostructures embedded in biosensors. However, the difficulty in real-world applications with analytical specificity is that molecular recognition on nanowires mainly depends on antibody modification with multistep modification procedures. Furthermore, the antibody modification suffers from nonspecific adsorption of undesired proteins in body fluid on the nanowires, which causes false responses and lowers sensitivity. Herein, we propose biomolecular recognition using surface-modified nanowires via thiolated 2-methacryloxyethyl phosphorylcholine (MPC-SH). MPC-SH enables self-assembled monolayer (SAM) modification, which contributes to the reduction of nonspecific adsorption of biomolecules onto the nanowires, and the specific capture of a target protein is attained in the presence of calcium ions. Our concept demonstrates the recognition of the biomarker protein on nanowire surfaces modified by MPC-SH SAM with a single step modification procedure.

Original languageEnglish
Pages (from-to)3225-3229
Number of pages5
JournalLab on a Chip
Volume18
Issue number21
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Nanowires
Self assembled monolayers
Molecular recognition
Biosensing Techniques
Proteins
Biosensors
Antibodies
Adsorption
Phosphorylcholine
Nanostructures
Body fluids
Body Fluids
Biomolecules
Biomarkers
Calcium
Ions

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Shimada, T., Yasui, T., Yokoyama, A., Goda, T., Hara, M., Yanagida, T., ... Baba, Y. (2018). Biomolecular recognition on nanowire surfaces modified by the self-assembled monolayer. Lab on a Chip, 18(21), 3225-3229. https://doi.org/10.1039/c8lc00438b

Biomolecular recognition on nanowire surfaces modified by the self-assembled monolayer. / Shimada, Taisuke; Yasui, Takao; Yokoyama, Asami; Goda, Tatsuro; Hara, Mitsuo; Yanagida, Takeshi; Kaji, Noritada; Kanai, Masaki; Nagashima, Kazuki; Miyahara, Yuji; Kawai, Tomoji; Baba, Yoshinobu.

In: Lab on a Chip, Vol. 18, No. 21, 01.01.2018, p. 3225-3229.

Research output: Contribution to journalArticle

Shimada, T, Yasui, T, Yokoyama, A, Goda, T, Hara, M, Yanagida, T, Kaji, N, Kanai, M, Nagashima, K, Miyahara, Y, Kawai, T & Baba, Y 2018, 'Biomolecular recognition on nanowire surfaces modified by the self-assembled monolayer', Lab on a Chip, vol. 18, no. 21, pp. 3225-3229. https://doi.org/10.1039/c8lc00438b
Shimada, Taisuke ; Yasui, Takao ; Yokoyama, Asami ; Goda, Tatsuro ; Hara, Mitsuo ; Yanagida, Takeshi ; Kaji, Noritada ; Kanai, Masaki ; Nagashima, Kazuki ; Miyahara, Yuji ; Kawai, Tomoji ; Baba, Yoshinobu. / Biomolecular recognition on nanowire surfaces modified by the self-assembled monolayer. In: Lab on a Chip. 2018 ; Vol. 18, No. 21. pp. 3225-3229.
@article{f2a1426c9afa42938029857759edd20a,
title = "Biomolecular recognition on nanowire surfaces modified by the self-assembled monolayer",
abstract = "Molecular recognition is one of the key factors in designing biosensors due to which nanowires functionalized with molecular recognition have attracted a lot of attention as promising candidates for nanostructures embedded in biosensors. However, the difficulty in real-world applications with analytical specificity is that molecular recognition on nanowires mainly depends on antibody modification with multistep modification procedures. Furthermore, the antibody modification suffers from nonspecific adsorption of undesired proteins in body fluid on the nanowires, which causes false responses and lowers sensitivity. Herein, we propose biomolecular recognition using surface-modified nanowires via thiolated 2-methacryloxyethyl phosphorylcholine (MPC-SH). MPC-SH enables self-assembled monolayer (SAM) modification, which contributes to the reduction of nonspecific adsorption of biomolecules onto the nanowires, and the specific capture of a target protein is attained in the presence of calcium ions. Our concept demonstrates the recognition of the biomarker protein on nanowire surfaces modified by MPC-SH SAM with a single step modification procedure.",
author = "Taisuke Shimada and Takao Yasui and Asami Yokoyama and Tatsuro Goda and Mitsuo Hara and Takeshi Yanagida and Noritada Kaji and Masaki Kanai and Kazuki Nagashima and Yuji Miyahara and Tomoji Kawai and Yoshinobu Baba",
year = "2018",
month = "1",
day = "1",
doi = "10.1039/c8lc00438b",
language = "English",
volume = "18",
pages = "3225--3229",
journal = "Lab on a Chip - Miniaturisation for Chemistry and Biology",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",
number = "21",

}

TY - JOUR

T1 - Biomolecular recognition on nanowire surfaces modified by the self-assembled monolayer

AU - Shimada, Taisuke

AU - Yasui, Takao

AU - Yokoyama, Asami

AU - Goda, Tatsuro

AU - Hara, Mitsuo

AU - Yanagida, Takeshi

AU - Kaji, Noritada

AU - Kanai, Masaki

AU - Nagashima, Kazuki

AU - Miyahara, Yuji

AU - Kawai, Tomoji

AU - Baba, Yoshinobu

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Molecular recognition is one of the key factors in designing biosensors due to which nanowires functionalized with molecular recognition have attracted a lot of attention as promising candidates for nanostructures embedded in biosensors. However, the difficulty in real-world applications with analytical specificity is that molecular recognition on nanowires mainly depends on antibody modification with multistep modification procedures. Furthermore, the antibody modification suffers from nonspecific adsorption of undesired proteins in body fluid on the nanowires, which causes false responses and lowers sensitivity. Herein, we propose biomolecular recognition using surface-modified nanowires via thiolated 2-methacryloxyethyl phosphorylcholine (MPC-SH). MPC-SH enables self-assembled monolayer (SAM) modification, which contributes to the reduction of nonspecific adsorption of biomolecules onto the nanowires, and the specific capture of a target protein is attained in the presence of calcium ions. Our concept demonstrates the recognition of the biomarker protein on nanowire surfaces modified by MPC-SH SAM with a single step modification procedure.

AB - Molecular recognition is one of the key factors in designing biosensors due to which nanowires functionalized with molecular recognition have attracted a lot of attention as promising candidates for nanostructures embedded in biosensors. However, the difficulty in real-world applications with analytical specificity is that molecular recognition on nanowires mainly depends on antibody modification with multistep modification procedures. Furthermore, the antibody modification suffers from nonspecific adsorption of undesired proteins in body fluid on the nanowires, which causes false responses and lowers sensitivity. Herein, we propose biomolecular recognition using surface-modified nanowires via thiolated 2-methacryloxyethyl phosphorylcholine (MPC-SH). MPC-SH enables self-assembled monolayer (SAM) modification, which contributes to the reduction of nonspecific adsorption of biomolecules onto the nanowires, and the specific capture of a target protein is attained in the presence of calcium ions. Our concept demonstrates the recognition of the biomarker protein on nanowire surfaces modified by MPC-SH SAM with a single step modification procedure.

UR - http://www.scopus.com/inward/record.url?scp=85055202122&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055202122&partnerID=8YFLogxK

U2 - 10.1039/c8lc00438b

DO - 10.1039/c8lc00438b

M3 - Article

C2 - 30264843

AN - SCOPUS:85055202122

VL - 18

SP - 3225

EP - 3229

JO - Lab on a Chip - Miniaturisation for Chemistry and Biology

JF - Lab on a Chip - Miniaturisation for Chemistry and Biology

SN - 1473-0197

IS - 21

ER -