Biosynthesis of estradiol-17β in the ovarian follicles of the red seabream Pagrus major during vitellogenesis

Kohei Ohta, Sonoko Yamaguchi, Akihiko Yamaguchi, Koichi Okuzawa, Koichiro Gen, Hirohiko Kagawa, Michiya Matsuyama

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The red seabream Pagrus major is a useful experimental fish for studying the endocrine control of oogenesis in teleosts. This study investigated the steroidogenic pathway for estradiol-17β (E2) biosynthesis in the ovarian follicles of red seabream. Intact follicles were isolated during vitellogenesis and incubated in vitro with different radiolabeled steroid precursors. When 17-hydroxyprogesterone (17-P), dehydroepiandrosterone (DHEA), or androstenedione (AD) were used as precursors, both testosterone (T) and estrone (E1) were synthesized by follicles, leading to estradiol-17β (E2) production. Serum steroid levels measured by enzyme-linked immunosorbent assay showed that T, E1, and E2 were present in the circulation at levels ranging from 1 ng/mL to 2 ng/mL throughout the day during the spawning season. In vitro conversion of E1 into E2, however, was 15.8-fold greater than T conversion into E2, suggesting that E2 is synthesized mainly via E1 rather than T. The results showed that E2 was synthesized from pregnenolone via 17-hydroxypregnenolone, DHEA, AD, and E1. Thus, the study demonstrated the complete steroidogenic E2 synthesis pathway in the ovarian follicles of red seabream, and revealed that E1 is the major precursor of E2.

Original languageEnglish
Pages (from-to)680-687
Number of pages8
JournalFisheries Science
Volume68
Issue number3
DOIs
Publication statusPublished - Jul 23 2002

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Pagrus major
vitellogenesis
ovarian follicles
steroid
estradiol
biosynthesis
prasterone
androstenedione
testosterone
teleost
steroids
serum
spawning
17-hydroxyprogesterone
assay
enzyme
pregnenolone
fold
estrone
oogenesis

All Science Journal Classification (ASJC) codes

  • Aquatic Science

Cite this

Biosynthesis of estradiol-17β in the ovarian follicles of the red seabream Pagrus major during vitellogenesis. / Ohta, Kohei; Yamaguchi, Sonoko; Yamaguchi, Akihiko; Okuzawa, Koichi; Gen, Koichiro; Kagawa, Hirohiko; Matsuyama, Michiya.

In: Fisheries Science, Vol. 68, No. 3, 23.07.2002, p. 680-687.

Research output: Contribution to journalArticle

Ohta, Kohei ; Yamaguchi, Sonoko ; Yamaguchi, Akihiko ; Okuzawa, Koichi ; Gen, Koichiro ; Kagawa, Hirohiko ; Matsuyama, Michiya. / Biosynthesis of estradiol-17β in the ovarian follicles of the red seabream Pagrus major during vitellogenesis. In: Fisheries Science. 2002 ; Vol. 68, No. 3. pp. 680-687.
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