Biosynthesis of steroids in ovarian follicles of red seabream, Pagrus major (Sparidae, Teleostei) during final oocyte maturation and the relative effectiveness of steroid metabolites for germinal vesicle breakdown in vitro

Kohei Ohta, Sonoko Yamaguchi, Akihiko Yamaguchi, Koichiro Gen, Koichi Okuzawa, Hirohiko Kagawa, Michiya Matsuyama

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Abstract

The steroid synthesis pathway in the ovarian follicles of the red seabream during final oocyte maturation (FOM) was investigated by incubating intact follicles with different radioactively labeled steroid precursors. During FOM, the steroidogenic shift from estradiol-17β to 20β-hydroxylated progestin production occurred mainly due to a combination of inactivation of C17,20-lyase and activation of 20β-hydroxysteroid dehydrogenase. Of the steroids produced, 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) and 17,20β,21-trihydroxy-4-pregnen-3-one (20β-S) exhibited the greatest effect on germinal vesicle breakdown (GVBD) in vitro. 17,20β-P was further converted to its 5β-reduced form, 17,20β-dihydroxy-5β-pregnan-3-one (17,20β-P-5β), which had lower GVBD activity, suggesting that 5β-reduction plays a role in the inactivation of the maturation-inducing ability of 17,20β-P. In contrast, no 5β-reduced metabolite of 20β-S was found. Serum levels of 17,20β-P and 20β-S, measured by ELISA, showed that circulating levels of both progestins increased during FOM, and 20β-S levels were approximately twice as high as 17,20β-P levels. This study clarified the complete steroidogenesis pathway during FOM in red seabream ovarian follicles and showed that two 20β-hydroxylated progestins, 17,20β-P and 20β-S, act as maturation-inducing hormones in this species. The catabolites of these two progestins and their physiological roles in reproduction are also discussed.

Original languageEnglish
Pages (from-to)45-54
Number of pages10
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume133
Issue number1
DOIs
Publication statusPublished - Sep 1 2002

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Sea Bream
Ovarian Follicle
Biosynthesis
Progestins
Metabolites
Oocytes
Steroids
20-Hydroxysteroid Dehydrogenases
Lyases
Reproduction
Estradiol
Chemical activation
Enzyme-Linked Immunosorbent Assay
Hormones
In Vitro Techniques
Serum

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Molecular Biology

Cite this

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title = "Biosynthesis of steroids in ovarian follicles of red seabream, Pagrus major (Sparidae, Teleostei) during final oocyte maturation and the relative effectiveness of steroid metabolites for germinal vesicle breakdown in vitro",
abstract = "The steroid synthesis pathway in the ovarian follicles of the red seabream during final oocyte maturation (FOM) was investigated by incubating intact follicles with different radioactively labeled steroid precursors. During FOM, the steroidogenic shift from estradiol-17β to 20β-hydroxylated progestin production occurred mainly due to a combination of inactivation of C17,20-lyase and activation of 20β-hydroxysteroid dehydrogenase. Of the steroids produced, 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) and 17,20β,21-trihydroxy-4-pregnen-3-one (20β-S) exhibited the greatest effect on germinal vesicle breakdown (GVBD) in vitro. 17,20β-P was further converted to its 5β-reduced form, 17,20β-dihydroxy-5β-pregnan-3-one (17,20β-P-5β), which had lower GVBD activity, suggesting that 5β-reduction plays a role in the inactivation of the maturation-inducing ability of 17,20β-P. In contrast, no 5β-reduced metabolite of 20β-S was found. Serum levels of 17,20β-P and 20β-S, measured by ELISA, showed that circulating levels of both progestins increased during FOM, and 20β-S levels were approximately twice as high as 17,20β-P levels. This study clarified the complete steroidogenesis pathway during FOM in red seabream ovarian follicles and showed that two 20β-hydroxylated progestins, 17,20β-P and 20β-S, act as maturation-inducing hormones in this species. The catabolites of these two progestins and their physiological roles in reproduction are also discussed.",
author = "Kohei Ohta and Sonoko Yamaguchi and Akihiko Yamaguchi and Koichiro Gen and Koichi Okuzawa and Hirohiko Kagawa and Michiya Matsuyama",
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T1 - Biosynthesis of steroids in ovarian follicles of red seabream, Pagrus major (Sparidae, Teleostei) during final oocyte maturation and the relative effectiveness of steroid metabolites for germinal vesicle breakdown in vitro

AU - Ohta, Kohei

AU - Yamaguchi, Sonoko

AU - Yamaguchi, Akihiko

AU - Gen, Koichiro

AU - Okuzawa, Koichi

AU - Kagawa, Hirohiko

AU - Matsuyama, Michiya

PY - 2002/9/1

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N2 - The steroid synthesis pathway in the ovarian follicles of the red seabream during final oocyte maturation (FOM) was investigated by incubating intact follicles with different radioactively labeled steroid precursors. During FOM, the steroidogenic shift from estradiol-17β to 20β-hydroxylated progestin production occurred mainly due to a combination of inactivation of C17,20-lyase and activation of 20β-hydroxysteroid dehydrogenase. Of the steroids produced, 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) and 17,20β,21-trihydroxy-4-pregnen-3-one (20β-S) exhibited the greatest effect on germinal vesicle breakdown (GVBD) in vitro. 17,20β-P was further converted to its 5β-reduced form, 17,20β-dihydroxy-5β-pregnan-3-one (17,20β-P-5β), which had lower GVBD activity, suggesting that 5β-reduction plays a role in the inactivation of the maturation-inducing ability of 17,20β-P. In contrast, no 5β-reduced metabolite of 20β-S was found. Serum levels of 17,20β-P and 20β-S, measured by ELISA, showed that circulating levels of both progestins increased during FOM, and 20β-S levels were approximately twice as high as 17,20β-P levels. This study clarified the complete steroidogenesis pathway during FOM in red seabream ovarian follicles and showed that two 20β-hydroxylated progestins, 17,20β-P and 20β-S, act as maturation-inducing hormones in this species. The catabolites of these two progestins and their physiological roles in reproduction are also discussed.

AB - The steroid synthesis pathway in the ovarian follicles of the red seabream during final oocyte maturation (FOM) was investigated by incubating intact follicles with different radioactively labeled steroid precursors. During FOM, the steroidogenic shift from estradiol-17β to 20β-hydroxylated progestin production occurred mainly due to a combination of inactivation of C17,20-lyase and activation of 20β-hydroxysteroid dehydrogenase. Of the steroids produced, 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) and 17,20β,21-trihydroxy-4-pregnen-3-one (20β-S) exhibited the greatest effect on germinal vesicle breakdown (GVBD) in vitro. 17,20β-P was further converted to its 5β-reduced form, 17,20β-dihydroxy-5β-pregnan-3-one (17,20β-P-5β), which had lower GVBD activity, suggesting that 5β-reduction plays a role in the inactivation of the maturation-inducing ability of 17,20β-P. In contrast, no 5β-reduced metabolite of 20β-S was found. Serum levels of 17,20β-P and 20β-S, measured by ELISA, showed that circulating levels of both progestins increased during FOM, and 20β-S levels were approximately twice as high as 17,20β-P levels. This study clarified the complete steroidogenesis pathway during FOM in red seabream ovarian follicles and showed that two 20β-hydroxylated progestins, 17,20β-P and 20β-S, act as maturation-inducing hormones in this species. The catabolites of these two progestins and their physiological roles in reproduction are also discussed.

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