Biotinylation of silicon and nickel surfaces and detection of streptavidin as biosensor

Hirokazu Seto, Chie Yamashita, Seiji Kamba, Takashi Kondo, Makoto Hasegawa, Mitsuhiro Matsuno, Yuichi Ogawa, Yu Hoshino, Yoshiko Miura

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22 Citations (Scopus)

Abstract

The availability of metal mesh device sensors has been investigated using surface-modified nickel mesh. Biotin was immobilized on the sensor surfaces consisting of silicon and nickel via a thiol-ene click reaction, known as the Michael addition reaction. Biotinylation on the maleimidated surface was confirmed by X-ray photoelectron spectroscopy. The binding of streptavidin to the biotinylated surfaces was evaluated using a quartz crystal microbalance and a metal mesh device sensor, with both techniques providing similar binding constant value. The recognition ability of the biotin immobilized using the thiol-maleimide method for streptavidin was comparable to that of biotin immobilized via several other methods. The adsorption of a biotin conjugate onto the streptavidin-immobilized surface via the biotin-streptavidin-biotin sandwich method was evaluated using a fluorescent microarray, with the results demonstrating that the biological activity of the streptavidin remained.

Original languageEnglish
Pages (from-to)9457-9463
Number of pages7
JournalLangmuir
Volume29
Issue number30
DOIs
Publication statusPublished - Jul 30 2013

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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    Seto, H., Yamashita, C., Kamba, S., Kondo, T., Hasegawa, M., Matsuno, M., Ogawa, Y., Hoshino, Y., & Miura, Y. (2013). Biotinylation of silicon and nickel surfaces and detection of streptavidin as biosensor. Langmuir, 29(30), 9457-9463. https://doi.org/10.1021/la401068n