Bitter taste receptor T2R1 activities were compatible with behavioral sensitivity to bitterness in chickens

Nozomi Hirose, Yuko Kawabata, Fuminori Kawabata, Shotaro Nishimura, Shoji Tabata

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Clarification of the mechanism of the sense of taste in chickens will provide information useful for creating and improving new feedstuffs for chickens, because the character of the taste receptors in oral tissues affects feeding behavior in animals. In this study, we focused on the sensitivity to bitterness in chickens. We cloned one of the bitter taste receptors, T2R1, from the chicken palate, constructed several biosensor-cells expressing chicken T2R1 (cT2R1), and determined a highly sensitive biosensor of cT2R1 among them. By using Ca2+ imaging methods, we identified two agonists of cT2R1, dextromethorphan (Dex) and diphenidol (Dip). Dex was a new agonist of cT2R1 that was more potent than Dip. In a behavioral drinking study, the intake volumes of solutions of these compounds were significantly lower than that of water in chickens. These aversive concentrations were identical to the concentrations that could activate cT2R1 in a cell-based assay. These results suggest that the cT2R1 activities induced by these agonists are linked to behavioral sensitivity to bitterness in chickens.

Original languageEnglish
Pages (from-to)464-468
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume460
Issue number2
DOIs
Publication statusPublished - May 1 2015

Fingerprint

Dextromethorphan
Biosensors
Chickens
Assays
Animals
Cells
Tissue
Imaging techniques
Water
Biosensing Techniques
diphenidol
Palate
Feeding Behavior
Drinking

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Bitter taste receptor T2R1 activities were compatible with behavioral sensitivity to bitterness in chickens. / Hirose, Nozomi; Kawabata, Yuko; Kawabata, Fuminori; Nishimura, Shotaro; Tabata, Shoji.

In: Biochemical and Biophysical Research Communications, Vol. 460, No. 2, 01.05.2015, p. 464-468.

Research output: Contribution to journalArticle

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