Block by 5-hydroxytryptamine of neuronal acetylcholine receptor channels expressed in Xenopus oocytes

Ken Nakazawa, Takami Akiyama, Kazuhide Inoue

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

1. Effects of 5-hydroxytryptamine (5-HT) on neuronal nicotinic acetylcholine (ACh) receptor channels were investigated by expressing cloned channel subunits in Xenopus oocytes. 2. When channels were expressed with a combination of α3 and β4 subunits, 5-HT (10 to 300 μM) reversibly inhibited an inward current activated by 100 μM ACh in a concentration-dependent manner. The inhibition was also observed when α3 subunit was combined with β2 subunit instead of β4 subunit, or β4 subunit was combined with α2 or α4-1 subunit instead of α3 subunit to express channels. 3. Compounds known to antagonize at 5-HT receptors (LY53857, metoclopramide and propranolol) exhibited an agonistic effect: they inhibited the ACh-activated current. 4. The results suggest that 5-HT inhibits recombinant neuronal nicotinic receptor channels through a binding-site distinct from conventional 5-HT receptors. The binding-site may not be attributed to a unique type of channel subunits.

Original languageEnglish
Pages (from-to)495-500
Number of pages6
JournalCellular and molecular neurobiology
Volume15
Issue number4
DOIs
Publication statusPublished - Aug 1 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience
  • Cell Biology

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