Abstract
Background - Therapeutic angiogenesis by delivery of vascular endothelial growth factor (VEGF) has attracted attention. However, the role and function of VEGF in experimental restenosis (neointimal formation) after vascular intraluminal injury have not been addressed. Methods and Results - We report herein that blockade of VEGF by soluble VEGF receptor 1 (sFlt-1) gene transfer attenuated neointimal formation after intraluminal injury in rabbits, rats, and mice. sFlt-1 gene transfer markedly attenuated the early vascular inflammation and proliferation and later neointimal formation. sFlt-1 gene transfer also inhibited increased expression of inflammatory factors such as monocyte chemoattractant protein-1 and VEGF. Intravascular VEGF gene transfer enhanced angiogenesis in the adventitia but did not reduce neointimal formation. Conclusions - Increased expression and activity of VEGF are essential in the development of experimental restenosis after intraluminal injury by recruiting monocyte-lineage cells.
| Original language | English |
|---|---|
| Pages (from-to) | 2444-2452 |
| Number of pages | 9 |
| Journal | Circulation |
| Volume | 110 |
| Issue number | 16 |
| DOIs | |
| Publication status | Published - Oct 19 2004 |
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All Science Journal Classification (ASJC) codes
- Cardiology and Cardiovascular Medicine
- Physiology (medical)
Cite this
Blockade of vascular endothelial growth factor suppresses experimental restenosis after intraluminal injury by inhibiting recruitment of monocyte lineage cells. / Ohtani, Kisho; Egashira, Kensuke; Hiasa, Ken Ichi; Zhao, Qingwei; Kitamoto, Shiro; Ishibashi, Minako; Usui, Makoto; Inoue, Shujiro; Yonemitsu, Yoshikazu; Sueishi, Katsuo; Sata, Masataka; Shibuya, Masabumi; Sunagawa, Kenji.
In: Circulation, Vol. 110, No. 16, 19.10.2004, p. 2444-2452.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Blockade of vascular endothelial growth factor suppresses experimental restenosis after intraluminal injury by inhibiting recruitment of monocyte lineage cells
AU - Ohtani, Kisho
AU - Egashira, Kensuke
AU - Hiasa, Ken Ichi
AU - Zhao, Qingwei
AU - Kitamoto, Shiro
AU - Ishibashi, Minako
AU - Usui, Makoto
AU - Inoue, Shujiro
AU - Yonemitsu, Yoshikazu
AU - Sueishi, Katsuo
AU - Sata, Masataka
AU - Shibuya, Masabumi
AU - Sunagawa, Kenji
PY - 2004/10/19
Y1 - 2004/10/19
N2 - Background - Therapeutic angiogenesis by delivery of vascular endothelial growth factor (VEGF) has attracted attention. However, the role and function of VEGF in experimental restenosis (neointimal formation) after vascular intraluminal injury have not been addressed. Methods and Results - We report herein that blockade of VEGF by soluble VEGF receptor 1 (sFlt-1) gene transfer attenuated neointimal formation after intraluminal injury in rabbits, rats, and mice. sFlt-1 gene transfer markedly attenuated the early vascular inflammation and proliferation and later neointimal formation. sFlt-1 gene transfer also inhibited increased expression of inflammatory factors such as monocyte chemoattractant protein-1 and VEGF. Intravascular VEGF gene transfer enhanced angiogenesis in the adventitia but did not reduce neointimal formation. Conclusions - Increased expression and activity of VEGF are essential in the development of experimental restenosis after intraluminal injury by recruiting monocyte-lineage cells.
AB - Background - Therapeutic angiogenesis by delivery of vascular endothelial growth factor (VEGF) has attracted attention. However, the role and function of VEGF in experimental restenosis (neointimal formation) after vascular intraluminal injury have not been addressed. Methods and Results - We report herein that blockade of VEGF by soluble VEGF receptor 1 (sFlt-1) gene transfer attenuated neointimal formation after intraluminal injury in rabbits, rats, and mice. sFlt-1 gene transfer markedly attenuated the early vascular inflammation and proliferation and later neointimal formation. sFlt-1 gene transfer also inhibited increased expression of inflammatory factors such as monocyte chemoattractant protein-1 and VEGF. Intravascular VEGF gene transfer enhanced angiogenesis in the adventitia but did not reduce neointimal formation. Conclusions - Increased expression and activity of VEGF are essential in the development of experimental restenosis after intraluminal injury by recruiting monocyte-lineage cells.
UR - http://www.scopus.com/inward/record.url?scp=6444243603&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=6444243603&partnerID=8YFLogxK
U2 - 10.1161/01.CIR.0000145123.85083.66
DO - 10.1161/01.CIR.0000145123.85083.66
M3 - Article
C2 - 15477409
AN - SCOPUS:6444243603
VL - 110
SP - 2444
EP - 2452
JO - Circulation
JF - Circulation
SN - 0009-7322
IS - 16
ER -