Blocking of monocyte chemoattractant protein-1 (MCP-1) activity attenuates the severity of acute pancreatitis in rats

Toshiaki Ishibashi, Haifeng Zhao, Ken Kawabe, Takamasa Oono, Kensuke Egashira, Koichi Suzuki, Hajime Nawata, Ryoichi Takayanagi, Tetsuhide Ito

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

Background. Monocyte chemoattractant protein-1 (MCP-1) has been shown to affect the progression of various inflammatory disorders, including pancreatitis. To investigate the role of MCP-1 in acute pancreatitis and to seek possible therapeutic means, we evaluated the effect of a plasmid expression vector containing a dominant-negative mutant MCP-1 gene (mMCP-1). Methods. Two rat models of acute pancreatitis were employed that used either cerulein (for mild pancreatitis) or a mixture of 5% taurocholic acid and trypsin (for severe pancreatitis). At 6 h after induction of acute pancreatitis with or without injection of mMCP-1, serum amylase levels and cytokine levels, as well as morphological evaluation of the pancreas, were determined. Survival rates were also evaluated. Results. Severe pancreatitis was significantly reduced by mMCP-1 injection. mMCP-1 decreased serum levels of amylase, IL-6, IL-10, and LDH, and improved the survival rate 48 h after disease onset. Histopathological changes of pancreas and lungs were also improved by mMCP-1. Conclusions. MCP-1 appears to be involved in the progression of severe forms of acute pancreatitis. Our data suggested that MCP-1 is a candidate as a therapeutic target to treat acute pancreatitis.

Original languageEnglish
Pages (from-to)79-85
Number of pages7
JournalJournal of gastroenterology
Volume43
Issue number1
DOIs
Publication statusPublished - Jan 2008

All Science Journal Classification (ASJC) codes

  • Gastroenterology

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