Blood-Compatible Polymer for Hepatocyte Culture with High Hepatocyte-Specific Functions toward Bioartificial Liver Development

Takashi Hoshiba, Takayuki Otaki, Eri Nemoto, Hiroka Maruyama, Masaru Tanaka

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The development of bioartificial liver (BAL) is expected because of the shortage of donor liver for transplantation. The substrates for BAL require the following criteria: (a) blood compatibility, (b) hepatocyte adhesiveness, and (c) the ability to maintain hepatocyte-specific functions. Here, we examined blood-compatible poly(2-methoxyethyl acrylate) (PMEA) and poly(tetrahydrofurfuryl acrylate) (PTHFA) (PTHFA) as the substrates for BAL. HepG2, a human hepatocyte model, could adhere on PMEA and PTHFA substrates. The spreading of HepG2 cells was suppressed on PMEA substrates because integrin contribution to cell adhesion on PMEA substrate was low and integrin signaling was not sufficiently activated. Hepatocyte-specific gene expression in HepG2 cells increased on PMEA substrate, whereas the expression decreased on PTHFA substrates due to the nuclear localization of Yes-associated protein (YAP). These results indicate that blood-compatible PMEA is suitable for BAL substrate. Also, PMEA is expected to be used to regulate cell functions for blood-contacting tissue engineering.

Original languageEnglish
Pages (from-to)18096-18103
Number of pages8
JournalACS Applied Materials and Interfaces
Volume7
Issue number32
DOIs
Publication statusPublished - Aug 19 2015

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Liver
Polymers
Blood
Substrates
Integrins
acrylic acid
Hepatocytes
Cell adhesion
Tissue engineering
Gene expression
Proteins

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

Blood-Compatible Polymer for Hepatocyte Culture with High Hepatocyte-Specific Functions toward Bioartificial Liver Development. / Hoshiba, Takashi; Otaki, Takayuki; Nemoto, Eri; Maruyama, Hiroka; Tanaka, Masaru.

In: ACS Applied Materials and Interfaces, Vol. 7, No. 32, 19.08.2015, p. 18096-18103.

Research output: Contribution to journalArticle

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