BmDJ-1 is a key regulator of oxidative modification in the development of the Silkworm, Bombyx mori

Hiroko Tabunoki, Hiroaki Ode, Yutaka Banno, Susumu Katsuma, Toru Shimada, Kazuei Mita, Kimiko Yamamoto, Ryoichi Sato, Reiko Ishii-Nozawa, Jun ichi Satoh

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Abstract

We cloned cDNA for the Bombyx mori DJ-1 protein (BmDJ-1) from the brains of larvae. BmDJ-1 is composed of 190 amino acids and encoded by 672 nucleotides. Northern blot analysis showed that BmDJ-1 is transcribed as a 756-bp mRNA and has one isoform. Reverse transcriptase (RT)-PCR experiments revealed that the BmDJ-1 was present in the brain, fatbody, Malpighian tubule, ovary and testis but present in only low amounts in the silkgland and hemocyte of day 4 fifth instar larvae. Immunological analysis demonstrated the presence of BmDJ-1 in the brain, midgut, fatbody, Malpighian tubule, testis and ovary from the larvae to the adult. We found that BmDJ-1 has a unique expression pattern through the fifth instar larval to adult developmental stage. We assessed the anti-oxidative function of BmDJ-1 using rotenone (ROT) in day 3 fifth instar larvae. Administration of ROT to day 3 fifth instar larvae, together with exogenous (BmNPV-BmDJ-1 infection for 4 days in advance) BmDJ-1, produced significantly lower 24-h mortality in BmDJ-1 groups than in the control. 2D-PAGE revealed an isoelectric point (pI) shift to an acidic form for BmDJ-1 in BmN4 cells upon ROT stimulus. Among the factors examined for their effects on expression level of BmDJ-1 in the hemolymph, nitric oxide (NO) concentration was identified based on dramatic developmental stage-dependent changes. Administration of isosorbide dinitrate (ISDN), which is an NO donor, to BmN4 cells produced increased expression of BmDJ-1 compared to the control. These results suggest that BmDJ-1 might control oxidative stress in the cell due to NO and serves as a development modulation factor in B. mori.

Original languageEnglish
Article numbere17683
JournalPloS one
Volume6
Issue number3
DOIs
Publication statusPublished - 2011

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Tabunoki, H., Ode, H., Banno, Y., Katsuma, S., Shimada, T., Mita, K., Yamamoto, K., Sato, R., Ishii-Nozawa, R., & Satoh, J. I. (2011). BmDJ-1 is a key regulator of oxidative modification in the development of the Silkworm, Bombyx mori. PloS one, 6(3), [e17683]. https://doi.org/10.1371/journal.pone.0017683