Bmp7 and lef1 are the downstream effectors of androgen signaling in androgen-induced sex characteristics development in Medaka

Yukiko Ogino, Ikumi Hirakawa, Keiji Inohaya, Eri Sumiya, Shinichi Miyagawa, Nancy Denslow, Gen Yamada, Norihisa Tatarazako, Taisen Iguchi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Androgens play key roles in the morphological specification of male type sex attractive and reproductive organs, whereas little is known about the developmental mechanisms of such secondary sex characters. Medaka offers a clue about sexual differentiation. They show a prominent masculine sexual character for appendage development, the formation of papillary processes in the anal fin, which has been induced in females by exogenous androgen exposure. This current study shows that the development of papillary processes is promoted by androgen-dependent augmentation of bone morphogenic protein 7 (Bmp7) and lymphoid enhancer-binding factor-1 (Lef1). Androgen receptor (AR) subtypes, ARα and ARβ, are expressed in the distal region of outgrowing bone nodules of developing papillary processes. Development of papillary processes concomitant with the induction of Bmp7 and Lef1 in the distal bone nodules by exposure to methyltestosterone was significantly suppressed by an antiandrogen, flutamide, in female medaka. When Bmp signaling was inhibited in methyltestosterone-exposed females by its inhibitor, dorsomorphin, Lef1 expression was suppressed accompanied by reduced proliferation in the distal bone nodules and retarded bone deposition. These observations indicate that androgendependent expressions of Bmp7 and Lef1 are required for the bone nodule outgrowth leading to the formation of these secondary sex characteristics in medaka. The formation of androgen-induced papillary processes may provide insights into the mechanisms regulating the specification of sexual features in vertebrates.

Original languageEnglish
Pages (from-to)449-462
Number of pages14
JournalEndocrinology
Volume155
Issue number2
DOIs
Publication statusPublished - Feb 1 2014
Externally publishedYes

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Oryzias
Sexual Development
Sex Characteristics
Androgens
Lymphoid Enhancer-Binding Factor 1
Bone and Bones
Androgen Receptors
Proteins
Methyltestosterone
Flutamide
Androgen Antagonists
Sex Differentiation
Vertebrates

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Bmp7 and lef1 are the downstream effectors of androgen signaling in androgen-induced sex characteristics development in Medaka. / Ogino, Yukiko; Hirakawa, Ikumi; Inohaya, Keiji; Sumiya, Eri; Miyagawa, Shinichi; Denslow, Nancy; Yamada, Gen; Tatarazako, Norihisa; Iguchi, Taisen.

In: Endocrinology, Vol. 155, No. 2, 01.02.2014, p. 449-462.

Research output: Contribution to journalArticle

Ogino, Y, Hirakawa, I, Inohaya, K, Sumiya, E, Miyagawa, S, Denslow, N, Yamada, G, Tatarazako, N & Iguchi, T 2014, 'Bmp7 and lef1 are the downstream effectors of androgen signaling in androgen-induced sex characteristics development in Medaka', Endocrinology, vol. 155, no. 2, pp. 449-462. https://doi.org/10.1210/en.2013-1507
Ogino, Yukiko ; Hirakawa, Ikumi ; Inohaya, Keiji ; Sumiya, Eri ; Miyagawa, Shinichi ; Denslow, Nancy ; Yamada, Gen ; Tatarazako, Norihisa ; Iguchi, Taisen. / Bmp7 and lef1 are the downstream effectors of androgen signaling in androgen-induced sex characteristics development in Medaka. In: Endocrinology. 2014 ; Vol. 155, No. 2. pp. 449-462.
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