Boi-ogi-to (TJ-20), a Kampo Formula, Suppresses the Inflammatory Bone Destruction and the Expression of Cytokines in the Synovia of Ankle Joints of Adjuvant Arthritic Rats

Xinwen Zhang, Hiro Take, Yicong Liu, Junjun Ni, Chunfu Deng, Baohong Zhao, Hiroshi Nakanishi, Jing He, Xu Yan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

TJ-20 is a formula consisting of 6 herbs that has been used in the clinical treatment of rheumatoid arthritis (RA) in China and Japan for centuries. However, scientific evidence of the effects of TJ-20 has not been established. In the present study, we focused on the therapeutic effects and investigated the main function of TJ-20 on adjuvant arthritis (AA), an animal model of RA, which was induced with complete Freund's adjuvant (CFA). TJ-20 was administered orally at 600 mg/kg once a day from 0, 7, and 10 days to 8 weeks after the CFA treatment. TJ-20 significantly ameliorated inflammatory progression and bone destruction in AA in a time-dependent manner. Furthermore, TJ-20 significantly reduced the increased changes in a number of macrophages and helper T cells. Moreover, TJ-20 suppressed the expression of TNF-α whereas it augmented the expression of IL-10 and attenuated Th1 cells responses in the synovia of the ankle joint. Therefore, TJ-20 regulated the expression of proinflammatory and anti-inflammatory cytokines in macrophages and Th1/Th2 balance in the synovia of ankle joints in AA rats. These results suggest the positive anti-inflammatory effect of TJ-20 and provide a scientific basis for the clinical use of TJ-20 for RA.

Original languageEnglish
Article number3679295
JournalEvidence-based Complementary and Alternative Medicine
Volume2017
DOIs
Publication statusPublished - Jan 1 2017

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Kampo Medicine
Experimental Arthritis
Ankle Joint
Synovial Fluid
Rheumatoid Arthritis
Freund's Adjuvant
Cytokines
Bone and Bones
Anti-Inflammatory Agents
Macrophages
Th1-Th2 Balance
Th1 Cells
Therapeutic Uses
Helper-Inducer T-Lymphocytes
Interleukin-10
China
Japan
Animal Models
Therapeutics

All Science Journal Classification (ASJC) codes

  • Complementary and alternative medicine

Cite this

Boi-ogi-to (TJ-20), a Kampo Formula, Suppresses the Inflammatory Bone Destruction and the Expression of Cytokines in the Synovia of Ankle Joints of Adjuvant Arthritic Rats. / Zhang, Xinwen; Take, Hiro; Liu, Yicong; Ni, Junjun; Deng, Chunfu; Zhao, Baohong; Nakanishi, Hiroshi; He, Jing; Yan, Xu.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2017, 3679295, 01.01.2017.

Research output: Contribution to journalArticle

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abstract = "TJ-20 is a formula consisting of 6 herbs that has been used in the clinical treatment of rheumatoid arthritis (RA) in China and Japan for centuries. However, scientific evidence of the effects of TJ-20 has not been established. In the present study, we focused on the therapeutic effects and investigated the main function of TJ-20 on adjuvant arthritis (AA), an animal model of RA, which was induced with complete Freund's adjuvant (CFA). TJ-20 was administered orally at 600 mg/kg once a day from 0, 7, and 10 days to 8 weeks after the CFA treatment. TJ-20 significantly ameliorated inflammatory progression and bone destruction in AA in a time-dependent manner. Furthermore, TJ-20 significantly reduced the increased changes in a number of macrophages and helper T cells. Moreover, TJ-20 suppressed the expression of TNF-α whereas it augmented the expression of IL-10 and attenuated Th1 cells responses in the synovia of the ankle joint. Therefore, TJ-20 regulated the expression of proinflammatory and anti-inflammatory cytokines in macrophages and Th1/Th2 balance in the synovia of ankle joints in AA rats. These results suggest the positive anti-inflammatory effect of TJ-20 and provide a scientific basis for the clinical use of TJ-20 for RA.",
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AU - Zhang, Xinwen

AU - Take, Hiro

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AU - Ni, Junjun

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AU - Zhao, Baohong

AU - Nakanishi, Hiroshi

AU - He, Jing

AU - Yan, Xu

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