Bone formation in a rat calvarial defect model after transplanting autogenous bone marrow with beta-tricalcium phosphate

Nobuaki Shirasu, Takaaki Ueno, Yasuhisa Hirata, Azumi Hirata, Toshimasa Kagawa, Miwa Kanou, Masako Sawaki, Mari Wakimoto, Akiko Ota, Hideto Imura, Tatsushi Matsumura, Tomohiro Yamada, Eiki Yamachika, Kazuo Sano

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In the present study, we evaluated the osteogenic potential of an autogenous bone marrow graft combined with beta-tricalcium phosphate (beta-TCP) in a rat calvarial bone defect model. The bone marrow harvested from the tibia of 7-week-old rats was grafted autogenously in a calvarial defect together with beta-TCP (=BTG group, n=16) or without beta-TCP (=BG group, n=16). Groups of animals were also treated with beta-TCP alone (=TG group, n=16) and control animals (n=8) received no graft implanted into the defect. We then observed the process of bone formation by histology, enzyme histochemistry and immunohistochemistry. Five days after grafting, in the BTG and BG groups, cell proliferation and osteogenic differentiation were observed. From 5 to 10 days after surgery, active Runx2, osteopontin (OPN), and TRAP- positive cells appeared in the BTG and BG groups. New bone formation started in the defect in both the BTG and BG groups. At 30 days after grafting, the BTG group showed new bone development and replacement of beta-TCP to fill the bone defect. New bone formation in the BTG group was significantly greater than in the BG group (P<0.01). The TG group showed no marked bone formation in the defect. The combination graft of bone marrow with beta-TCP showed marked bone formation in rat calvarial defects. Our results indicate that the combination grafts of bone marrow with beta-TCP may be an effective technique for repairing bone defects Beta-TCPgraft (TG) group.

Original languageEnglish
Pages (from-to)270-277
Number of pages8
JournalActa Histochemica
Volume112
Issue number3
DOIs
Publication statusPublished - May 1 2010

Fingerprint

Osteogenesis
Bone Marrow
Transplants
Bone and Bones
Osteopontin
Bone Development
beta-tricalcium phosphate
Tibia
Ambulatory Surgical Procedures
Histology
Immunohistochemistry
Cell Proliferation
Enzymes

All Science Journal Classification (ASJC) codes

  • Histology
  • Cell Biology

Cite this

Bone formation in a rat calvarial defect model after transplanting autogenous bone marrow with beta-tricalcium phosphate. / Shirasu, Nobuaki; Ueno, Takaaki; Hirata, Yasuhisa; Hirata, Azumi; Kagawa, Toshimasa; Kanou, Miwa; Sawaki, Masako; Wakimoto, Mari; Ota, Akiko; Imura, Hideto; Matsumura, Tatsushi; Yamada, Tomohiro; Yamachika, Eiki; Sano, Kazuo.

In: Acta Histochemica, Vol. 112, No. 3, 01.05.2010, p. 270-277.

Research output: Contribution to journalArticle

Shirasu, N, Ueno, T, Hirata, Y, Hirata, A, Kagawa, T, Kanou, M, Sawaki, M, Wakimoto, M, Ota, A, Imura, H, Matsumura, T, Yamada, T, Yamachika, E & Sano, K 2010, 'Bone formation in a rat calvarial defect model after transplanting autogenous bone marrow with beta-tricalcium phosphate', Acta Histochemica, vol. 112, no. 3, pp. 270-277. https://doi.org/10.1016/j.acthis.2009.01.003
Shirasu, Nobuaki ; Ueno, Takaaki ; Hirata, Yasuhisa ; Hirata, Azumi ; Kagawa, Toshimasa ; Kanou, Miwa ; Sawaki, Masako ; Wakimoto, Mari ; Ota, Akiko ; Imura, Hideto ; Matsumura, Tatsushi ; Yamada, Tomohiro ; Yamachika, Eiki ; Sano, Kazuo. / Bone formation in a rat calvarial defect model after transplanting autogenous bone marrow with beta-tricalcium phosphate. In: Acta Histochemica. 2010 ; Vol. 112, No. 3. pp. 270-277.
@article{55b4b0849d2a45598d4bf0a9f08d5321,
title = "Bone formation in a rat calvarial defect model after transplanting autogenous bone marrow with beta-tricalcium phosphate",
abstract = "In the present study, we evaluated the osteogenic potential of an autogenous bone marrow graft combined with beta-tricalcium phosphate (beta-TCP) in a rat calvarial bone defect model. The bone marrow harvested from the tibia of 7-week-old rats was grafted autogenously in a calvarial defect together with beta-TCP (=BTG group, n=16) or without beta-TCP (=BG group, n=16). Groups of animals were also treated with beta-TCP alone (=TG group, n=16) and control animals (n=8) received no graft implanted into the defect. We then observed the process of bone formation by histology, enzyme histochemistry and immunohistochemistry. Five days after grafting, in the BTG and BG groups, cell proliferation and osteogenic differentiation were observed. From 5 to 10 days after surgery, active Runx2, osteopontin (OPN), and TRAP- positive cells appeared in the BTG and BG groups. New bone formation started in the defect in both the BTG and BG groups. At 30 days after grafting, the BTG group showed new bone development and replacement of beta-TCP to fill the bone defect. New bone formation in the BTG group was significantly greater than in the BG group (P<0.01). The TG group showed no marked bone formation in the defect. The combination graft of bone marrow with beta-TCP showed marked bone formation in rat calvarial defects. Our results indicate that the combination grafts of bone marrow with beta-TCP may be an effective technique for repairing bone defects Beta-TCPgraft (TG) group.",
author = "Nobuaki Shirasu and Takaaki Ueno and Yasuhisa Hirata and Azumi Hirata and Toshimasa Kagawa and Miwa Kanou and Masako Sawaki and Mari Wakimoto and Akiko Ota and Hideto Imura and Tatsushi Matsumura and Tomohiro Yamada and Eiki Yamachika and Kazuo Sano",
year = "2010",
month = "5",
day = "1",
doi = "10.1016/j.acthis.2009.01.003",
language = "English",
volume = "112",
pages = "270--277",
journal = "Acta Histochemica",
issn = "0065-1281",
publisher = "Urban und Fischer Verlag Jena",
number = "3",

}

TY - JOUR

T1 - Bone formation in a rat calvarial defect model after transplanting autogenous bone marrow with beta-tricalcium phosphate

AU - Shirasu, Nobuaki

AU - Ueno, Takaaki

AU - Hirata, Yasuhisa

AU - Hirata, Azumi

AU - Kagawa, Toshimasa

AU - Kanou, Miwa

AU - Sawaki, Masako

AU - Wakimoto, Mari

AU - Ota, Akiko

AU - Imura, Hideto

AU - Matsumura, Tatsushi

AU - Yamada, Tomohiro

AU - Yamachika, Eiki

AU - Sano, Kazuo

PY - 2010/5/1

Y1 - 2010/5/1

N2 - In the present study, we evaluated the osteogenic potential of an autogenous bone marrow graft combined with beta-tricalcium phosphate (beta-TCP) in a rat calvarial bone defect model. The bone marrow harvested from the tibia of 7-week-old rats was grafted autogenously in a calvarial defect together with beta-TCP (=BTG group, n=16) or without beta-TCP (=BG group, n=16). Groups of animals were also treated with beta-TCP alone (=TG group, n=16) and control animals (n=8) received no graft implanted into the defect. We then observed the process of bone formation by histology, enzyme histochemistry and immunohistochemistry. Five days after grafting, in the BTG and BG groups, cell proliferation and osteogenic differentiation were observed. From 5 to 10 days after surgery, active Runx2, osteopontin (OPN), and TRAP- positive cells appeared in the BTG and BG groups. New bone formation started in the defect in both the BTG and BG groups. At 30 days after grafting, the BTG group showed new bone development and replacement of beta-TCP to fill the bone defect. New bone formation in the BTG group was significantly greater than in the BG group (P<0.01). The TG group showed no marked bone formation in the defect. The combination graft of bone marrow with beta-TCP showed marked bone formation in rat calvarial defects. Our results indicate that the combination grafts of bone marrow with beta-TCP may be an effective technique for repairing bone defects Beta-TCPgraft (TG) group.

AB - In the present study, we evaluated the osteogenic potential of an autogenous bone marrow graft combined with beta-tricalcium phosphate (beta-TCP) in a rat calvarial bone defect model. The bone marrow harvested from the tibia of 7-week-old rats was grafted autogenously in a calvarial defect together with beta-TCP (=BTG group, n=16) or without beta-TCP (=BG group, n=16). Groups of animals were also treated with beta-TCP alone (=TG group, n=16) and control animals (n=8) received no graft implanted into the defect. We then observed the process of bone formation by histology, enzyme histochemistry and immunohistochemistry. Five days after grafting, in the BTG and BG groups, cell proliferation and osteogenic differentiation were observed. From 5 to 10 days after surgery, active Runx2, osteopontin (OPN), and TRAP- positive cells appeared in the BTG and BG groups. New bone formation started in the defect in both the BTG and BG groups. At 30 days after grafting, the BTG group showed new bone development and replacement of beta-TCP to fill the bone defect. New bone formation in the BTG group was significantly greater than in the BG group (P<0.01). The TG group showed no marked bone formation in the defect. The combination graft of bone marrow with beta-TCP showed marked bone formation in rat calvarial defects. Our results indicate that the combination grafts of bone marrow with beta-TCP may be an effective technique for repairing bone defects Beta-TCPgraft (TG) group.

UR - http://www.scopus.com/inward/record.url?scp=77950342110&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950342110&partnerID=8YFLogxK

U2 - 10.1016/j.acthis.2009.01.003

DO - 10.1016/j.acthis.2009.01.003

M3 - Article

C2 - 19403161

AN - SCOPUS:77950342110

VL - 112

SP - 270

EP - 277

JO - Acta Histochemica

JF - Acta Histochemica

SN - 0065-1281

IS - 3

ER -