Bone-like nodules formed in vitro by rat periodontal ligament cells

M. Mukai, Y. Yoshimine, A. Akamine, K. Maeda

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The periodontal ligament has been shown to possess the ability to regenerate both new cementum and alveolar bone as well as a self-regenerative capacity; however, the source of cementoblasts and osteoblasts is not still clear. We investigated the development of bone-like tissue in vitro by periodontal ligament cells, in order to determine whether the periodontal ligament contains osteoprogenitor cells. Periodontal ligament cells were obtained from periodontal ligament tissue attached to the maxillary incisors of 6-week-old WKA rats by means of the explant technique. Cells at passage #3 were cultured for long term in α-minimum essential medium containing 10% fetal bovine serum, antibiotics, and 50 μg/ml ascorbic acid, and were then examined using phase-contrast microscopy, histochemistry, transmission electron microscopy, X-ray microanalysis, and electron diffraction. Nodules were formed in the cultures, and when 10 mM Na-β-glycerophosphate was added, these nodules became mineralized. The mineralized nodules were identified as bone-like elements in view of the presence of osteoblast-like and osteocyte-like cells, collagenous matrix, a mineral composed of hydroxyapatite, and intense alkaline phosphatase activity. The results show that the periodontal ligament contains osteoprogenitor cells, which differentiate into osteoblasts and produce bone-like tissue.

Original languageEnglish
Pages (from-to)453-460
Number of pages8
JournalCell & Tissue Research
Volume271
Issue number3
DOIs
Publication statusPublished - Mar 1 1993

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Periodontal Ligament
Bone and Bones
Osteoblasts
Dental Cementum
Glycerophosphates
Electron Probe Microanalysis
Phase-Contrast Microscopy
Osteocytes
Incisor
Durapatite
Transmission Electron Microscopy
Ascorbic Acid
Minerals
Alkaline Phosphatase
In Vitro Techniques
Electrons
Anti-Bacterial Agents
Serum

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Bone-like nodules formed in vitro by rat periodontal ligament cells. / Mukai, M.; Yoshimine, Y.; Akamine, A.; Maeda, K.

In: Cell & Tissue Research, Vol. 271, No. 3, 01.03.1993, p. 453-460.

Research output: Contribution to journalArticle

Mukai, M. ; Yoshimine, Y. ; Akamine, A. ; Maeda, K. / Bone-like nodules formed in vitro by rat periodontal ligament cells. In: Cell & Tissue Research. 1993 ; Vol. 271, No. 3. pp. 453-460.
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