TY - JOUR
T1 - Bone marrow and peripheral blood expression of ID1 in human gastric carcinoma patients is a bona fide indicator of lymph node and peritoneal metastasis
AU - Iwatsuki, M.
AU - Fukagawa, T.
AU - Mimori, K.
AU - Nakanishi, H.
AU - Ito, S.
AU - Ishii, H.
AU - Yokobori, T.
AU - Sasako, M.
AU - Baba, H.
AU - Mori, M.
N1 - Funding Information:
We thank T Shimooka, K Ogata, M Kasagi, Y Nakagawa and T Kawano for their technical assistance. This work was supported in part by the following grants and foundations: CREST, Japan Science and Technology Agency (JST); Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Scientific Research, grant numbers 17109013, 18659384, 18390367, 18590333, 18015039, 19591509, 19390336, 20390360, 20591547, 20790961 and 20790960; The Ministry of Education, Culture, Sports, Science and Technology (MEXT) Grant-in-Aid for Scientific Research on Priority Areas, grant number 18015039; Third Term Comprehensive Ten-year Strategy for Cancer Control, grant number 16271201; NEDO (New Energy and Industrial Technology Development Organization) Technological Development for Chromosome Analysis.
PY - 2009/6/16
Y1 - 2009/6/16
N2 - Recent studies have showed that the bone marrow-derived endothelial progenitor cells play critical roles in metastasis and that ID1 is required in metastasis as regulator of angiogenesis. Therefore, we investigated the clinical significance of ID1 mRNA expression in bone marrow and peripheral samples in patients with gastric cancer. Two hundred and eighty-nine bone marrow and 196 peripheral blood samples from gastric cancer patients were collected and analysed by quantitative RT-PCR for ID1. The ID1 protein expression in one bone marrow, three metastatic lymph nodes and three peritoneal disseminated tumours was examined by immunohistochemical methods. In both bone marrow and peripheral blood samples, ID1 mRNA expression in the metastatic group was significantly higher than in any other group (P0.003, P0.0001, respectively) and significantly associated with lymph node metastasis and peritoneal dissemination. The cells in bone marrow with metastatic cancer stained strongly with ID1 compared with those of healthy volunteers. The expression of ID1 mRNA in bone marrow and peripheral blood was significantly associated with lymph node metastasis and peritoneal dissemination, and therefore constitutes a predictable marker for lymph node metastasis and peritoneal dissemination.
AB - Recent studies have showed that the bone marrow-derived endothelial progenitor cells play critical roles in metastasis and that ID1 is required in metastasis as regulator of angiogenesis. Therefore, we investigated the clinical significance of ID1 mRNA expression in bone marrow and peripheral samples in patients with gastric cancer. Two hundred and eighty-nine bone marrow and 196 peripheral blood samples from gastric cancer patients were collected and analysed by quantitative RT-PCR for ID1. The ID1 protein expression in one bone marrow, three metastatic lymph nodes and three peritoneal disseminated tumours was examined by immunohistochemical methods. In both bone marrow and peripheral blood samples, ID1 mRNA expression in the metastatic group was significantly higher than in any other group (P0.003, P0.0001, respectively) and significantly associated with lymph node metastasis and peritoneal dissemination. The cells in bone marrow with metastatic cancer stained strongly with ID1 compared with those of healthy volunteers. The expression of ID1 mRNA in bone marrow and peripheral blood was significantly associated with lymph node metastasis and peritoneal dissemination, and therefore constitutes a predictable marker for lymph node metastasis and peritoneal dissemination.
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U2 - 10.1038/sj.bjc.6605085
DO - 10.1038/sj.bjc.6605085
M3 - Article
C2 - 19491902
AN - SCOPUS:67349230926
SN - 0007-0920
VL - 100
SP - 1937
EP - 1942
JO - British Journal of Cancer
JF - British Journal of Cancer
IS - 12
ER -
