Bone marrow-derived cells are essential for intrathymic deletion of self-reactive T cells in both the host- and donor-derived thymocytes of fully allogeneic bone marrow chimeras

Y. Yoshikai, M. Ogimoto, G. Matsuzaki, K. Nomoto

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    The fate of self-reactive T cells was examined in both the host- and donor-derived thymocytes of fully allogeneic bone marrow (BM) chimeras of two strain combinations of AKR/J (H-2(k), IE+, Thy-1.1, Mls-1a2b) and C57BL/6 (H-2b, IE-, Thy-1.2, Mls-1b2b). Sequential appearance of host- and donor-derived T cells occurred in the thymus of both AKR → B6 and B6 → AKR chimeras in which 5 x 106 of T cell-depleted BM cells were used to reconstitute recipients lethally irradiated with 950 rad. Thymocytes bearing Vβ6(high), which recognize MHC class II IE-binding Ag encoded by Mls-1a allele, were detected in neither host- nor donor-derived thymocytes of AKR-B6 chimeras in which Mls-1a and IE were expressed only by the BM-derived cells. Thymocytes bearing Vβ11(high) capable of recognizing IE were also deleted in the host- and donor-derived thymocytes of the AKR → B6 chimeras. One million of BM cells were inadequate to deletion of the Vβ6(high) and Vβ11(high) T cells in the host-derived thymocytes of these chimeras. On the other hand, significant numbers of Vβ6(high) and Vβ11(high) thymocytes were detected in both the host- and donor-derived thymocytes in B6 → AKR chimeras where sufficient doses of IE- stem cells were used to reconstitute irradiated Mls-1aIE+ recipients. These results suggest that clonal deletion of the host- and donor-reactive T cells in both the host- and donor-derived thymocytes is an important mechanism for the induction of transplantation tolerance in allogeneic BM chimeras and that BM-derived APC may be essential for the intrathymic elimination of both the host- and donor-reactive T cells in the BM chimeras.

    Original languageEnglish
    Pages (from-to)505-509
    Number of pages5
    JournalJournal of Immunology
    Volume145
    Issue number2
    Publication statusPublished - 1990

    All Science Journal Classification (ASJC) codes

    • Immunology

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