Bone marrow-derived cells in the population of spinal microglia after peripheral nerve injury

Ryoichi Tashima, Satsuki Mikuriya, Daisuke Tomiyama, Miho Shiratori-Hayashi, Tomohiro Yamashita, Yuta Kohro, Hidetoshi Tozaki-Saitoh, Kazuhide Inoue, Makoto Tsuda

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Abstract

Accumulating evidence indicates that peripheral nerve injury (PNI) activates spinal microglia that are necessary for neuropathic pain. Recent studies using bone marrow (BM) chimeric mice have reported that after PNI, circulating BM-derived cells infiltrate into the spinal cord and differentiate into microglia-like cells. This raises the possibility that the population of spinal microglia after PNI may be heterogeneous. However, the infiltration of BM cells in the spinal cord remains controversial because of experimental adverse effects of strong irradiation used for generating BM chimeric mice. In this study, we evaluated the PNI-induced spinal infiltration of BM-derived cells not only by irradiation-induced myeloablation with various conditioning regimens, but also by parabiosis and mice with genetically labelled microglia, models without irradiation and BM transplantation. Results obtained from these independent approaches provide compelling evidence indicating little contribution of circulating BM-derived cells to the population of spinal microglia after PNI.

Original languageEnglish
Article number23701
JournalScientific reports
Volume6
DOIs
Publication statusPublished - Mar 23 2016

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Peripheral Nerve Injuries
Microglia
Bone Marrow Cells
Population
Spinal Cord
Parabiosis
Bone Marrow
Neuralgia
Bone Marrow Transplantation

All Science Journal Classification (ASJC) codes

  • General

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Bone marrow-derived cells in the population of spinal microglia after peripheral nerve injury. / Tashima, Ryoichi; Mikuriya, Satsuki; Tomiyama, Daisuke; Shiratori-Hayashi, Miho; Yamashita, Tomohiro; Kohro, Yuta; Tozaki-Saitoh, Hidetoshi; Inoue, Kazuhide; Tsuda, Makoto.

In: Scientific reports, Vol. 6, 23701, 23.03.2016.

Research output: Contribution to journalArticle

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