Boron nutrition of cultured tobacco BY-2 cells. VII. Rapid induction of metabolic dysfunction in cells deprived of boron as revealed by microarray analysis

Taichi Koshiba, Masaru Kobayashi, Ken Matsuoka, Toru Fujiwara, Toru Matoh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In plants, boron (B) works to build the cell wall structure by forming borate ester cross-links with pectin, and the deficiency of this element brings about oxidative damage and cell death. However, the process via which B deficiency leads to such catastrophe has not been well understood. In this study, we investigated the temporal changes in transcript profiles of B-deprived cultured tobacco (Nicotiana tabacum L.) BY-2 cells using cDNA microarray. The results suggest that the cells deprived of B up-regulated their antioxidative defense system to cope with the stress condition. However, the adaptive responses seemed to have declined within the first 12 h of deprivation. Tetrazolium reducing activity of B-deprived cells was lower than that of control cells at 12 h. These results together suggest that cellular function in B-deprived cells was seriously damaged as early as 12 h after deprivation. The rapidity of the onset of B deprivation-induced damages suggests the importance of the pectic network covalently cross-linked with B.

Original languageEnglish
Pages (from-to)189-194
Number of pages6
JournalSoil Science and Plant Nutrition
Volume59
Issue number2
DOIs
Publication statusPublished - Apr 2013

All Science Journal Classification (ASJC) codes

  • Soil Science
  • Plant Science

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