Both RadA and RadB are involved in homologous recombination in Pyrococcus furiosus

Kayoko Komori, Tomoko Miyata, Jocelyne DiRuggiero, Rhonda Holley-Shanks, Ikuko Hayashi, Isaac K.O. Cann, Kota Mayanagi, Hideo Shinagawa, Yoshizumi Ishino

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

RecA and Rad51 proteins are essential for homologous recombination in Bacteria and Eukarya, respectively. Homologous proteins, called RadA, have been described for Archaea. Here we present the characterization of two RecA/Rad51 family proteins, RadA and RadB, from Pyrococcus furiosus. The radA and radB genes were not induced by DNA damage resulting from exposure of the cells to γ and UV irradiation and heat shock, suggesting that they might be constitutively expressed in this hyperthermophile. RadA had DNA-dependent ATPase, D. loop formation, and strand exchange activities. In contrast, RadB had a very weak ATPase activity that is not stimulated by DNA. This protein had a strong binding affinity for DNA, but little strand exchange activity could be detected. A direct interaction between RadA and RadB was detected by an immunoprecipitation assay. Moreover, RadB, but not RadA, coprecipitated with Hjc, a Holliday junction resolvase found in P. furiosus, in the absence of ATP. This interaction was suppressed in the presence of ATP. The Holliday junction cleavage activity of Hjc was inhibited by RadB in the absence, but not in the presence, of ATP. These results suggest that RadB has important roles in homologous recombination in Archaea and may regulate the cleavage reactions of the branch-structured DNA.

Original languageEnglish
Pages (from-to)33782-33790
Number of pages9
JournalJournal of Biological Chemistry
Volume275
Issue number43
DOIs
Publication statusPublished - Oct 27 2000
Externally publishedYes

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Pyrococcus furiosus
Homologous Recombination
Adenosine Triphosphate
Archaea
Adenosine Triphosphatases
Holliday Junction Resolvases
DNA
Cruciform DNA
Rec A Recombinases
Proteins
Eukaryota
Immunoprecipitation
DNA Damage
Shock
Hot Temperature
Bacteria
Assays
Genes
Irradiation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Komori, K., Miyata, T., DiRuggiero, J., Holley-Shanks, R., Hayashi, I., Cann, I. K. O., ... Ishino, Y. (2000). Both RadA and RadB are involved in homologous recombination in Pyrococcus furiosus. Journal of Biological Chemistry, 275(43), 33782-33790. https://doi.org/10.1074/jbc.M004557200

Both RadA and RadB are involved in homologous recombination in Pyrococcus furiosus. / Komori, Kayoko; Miyata, Tomoko; DiRuggiero, Jocelyne; Holley-Shanks, Rhonda; Hayashi, Ikuko; Cann, Isaac K.O.; Mayanagi, Kota; Shinagawa, Hideo; Ishino, Yoshizumi.

In: Journal of Biological Chemistry, Vol. 275, No. 43, 27.10.2000, p. 33782-33790.

Research output: Contribution to journalArticle

Komori, K, Miyata, T, DiRuggiero, J, Holley-Shanks, R, Hayashi, I, Cann, IKO, Mayanagi, K, Shinagawa, H & Ishino, Y 2000, 'Both RadA and RadB are involved in homologous recombination in Pyrococcus furiosus', Journal of Biological Chemistry, vol. 275, no. 43, pp. 33782-33790. https://doi.org/10.1074/jbc.M004557200
Komori K, Miyata T, DiRuggiero J, Holley-Shanks R, Hayashi I, Cann IKO et al. Both RadA and RadB are involved in homologous recombination in Pyrococcus furiosus. Journal of Biological Chemistry. 2000 Oct 27;275(43):33782-33790. https://doi.org/10.1074/jbc.M004557200
Komori, Kayoko ; Miyata, Tomoko ; DiRuggiero, Jocelyne ; Holley-Shanks, Rhonda ; Hayashi, Ikuko ; Cann, Isaac K.O. ; Mayanagi, Kota ; Shinagawa, Hideo ; Ishino, Yoshizumi. / Both RadA and RadB are involved in homologous recombination in Pyrococcus furiosus. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 43. pp. 33782-33790.
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abstract = "RecA and Rad51 proteins are essential for homologous recombination in Bacteria and Eukarya, respectively. Homologous proteins, called RadA, have been described for Archaea. Here we present the characterization of two RecA/Rad51 family proteins, RadA and RadB, from Pyrococcus furiosus. The radA and radB genes were not induced by DNA damage resulting from exposure of the cells to γ and UV irradiation and heat shock, suggesting that they might be constitutively expressed in this hyperthermophile. RadA had DNA-dependent ATPase, D. loop formation, and strand exchange activities. In contrast, RadB had a very weak ATPase activity that is not stimulated by DNA. This protein had a strong binding affinity for DNA, but little strand exchange activity could be detected. A direct interaction between RadA and RadB was detected by an immunoprecipitation assay. Moreover, RadB, but not RadA, coprecipitated with Hjc, a Holliday junction resolvase found in P. furiosus, in the absence of ATP. This interaction was suppressed in the presence of ATP. The Holliday junction cleavage activity of Hjc was inhibited by RadB in the absence, but not in the presence, of ATP. These results suggest that RadB has important roles in homologous recombination in Archaea and may regulate the cleavage reactions of the branch-structured DNA.",
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AU - Hayashi, Ikuko

AU - Cann, Isaac K.O.

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