Bradykinin activates ADP-ribosyl cyclase in neuroblastoma cells: Intracellular concentration decrease in NAD and increase in cyclic ADP-ribose

Haruhiro Higashida; Alla Salmina; Minako Hashii; Shigeru Yokoyama; Jia Sheng Zhang; Mami Noda; Zen Guo Zhong; Duo Jin

doi:10.1016/j.febslet.2006.07.084

Bradykinin activates ADP-ribosyl cyclase in neuroblastoma cells: Intracellular concentration decrease in NAD and increase in cyclic ADP-ribose

Haruhiro Higashida, Alla Salmina, Minako Hashii, Shigeru Yokoyama, Jia Sheng Zhang, Mami Noda, Zen Guo Zhong, Duo Jin

Pharmaceutical Health Care and Sciences Faculty

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

ADP-ribosyl cyclase activity in the crude membrane fraction of neuroblastoma × glioma NGPM1-27 hybrid cells was measured by monitoring [³H] cyclic ADP-ribose (cADPR) formation from [³H] NAD⁺. Bradykinin (BK) at 100 nM increased ADP-ribosyl cyclase activity by about 2.5-fold. Application of 300 nM BK to living NGPM1-27 cells decreased NAD⁺ to 78% of the prestimulation level at 30 s. In contrast, intracellular cADPR concentrations were increased by 2-3-fold during the period from 30 to 120 s after the same treatment. Our results suggest that cADPR is one of the second messengers downstream of B₂ BK receptors.

Original language	English
Pages (from-to)	4857-4860
Number of pages	4
Journal	FEBS Letters
Volume	580
Issue number	20
DOIs	https://doi.org/10.1016/j.febslet.2006.07.084
Publication status	Published - Sept 4 2006

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2006.07.084

Cite this

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title = "Bradykinin activates ADP-ribosyl cyclase in neuroblastoma cells: Intracellular concentration decrease in NAD and increase in cyclic ADP-ribose",

abstract = "ADP-ribosyl cyclase activity in the crude membrane fraction of neuroblastoma × glioma NGPM1-27 hybrid cells was measured by monitoring [3H] cyclic ADP-ribose (cADPR) formation from [3H] NAD+. Bradykinin (BK) at 100 nM increased ADP-ribosyl cyclase activity by about 2.5-fold. Application of 300 nM BK to living NGPM1-27 cells decreased NAD+ to 78% of the prestimulation level at 30 s. In contrast, intracellular cADPR concentrations were increased by 2-3-fold during the period from 30 to 120 s after the same treatment. Our results suggest that cADPR is one of the second messengers downstream of B2 BK receptors.",

author = "Haruhiro Higashida and Alla Salmina and Minako Hashii and Shigeru Yokoyama and Zhang, {Jia Sheng} and Mami Noda and Zhong, {Zen Guo} and Duo Jin",

year = "2006",

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doi = "10.1016/j.febslet.2006.07.084",

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T1 - Bradykinin activates ADP-ribosyl cyclase in neuroblastoma cells

T2 - Intracellular concentration decrease in NAD and increase in cyclic ADP-ribose

AU - Higashida, Haruhiro

AU - Salmina, Alla

AU - Hashii, Minako

AU - Yokoyama, Shigeru

AU - Zhang, Jia Sheng

AU - Noda, Mami

AU - Zhong, Zen Guo

AU - Jin, Duo

PY - 2006/9/4

Y1 - 2006/9/4

N2 - ADP-ribosyl cyclase activity in the crude membrane fraction of neuroblastoma × glioma NGPM1-27 hybrid cells was measured by monitoring [3H] cyclic ADP-ribose (cADPR) formation from [3H] NAD+. Bradykinin (BK) at 100 nM increased ADP-ribosyl cyclase activity by about 2.5-fold. Application of 300 nM BK to living NGPM1-27 cells decreased NAD+ to 78% of the prestimulation level at 30 s. In contrast, intracellular cADPR concentrations were increased by 2-3-fold during the period from 30 to 120 s after the same treatment. Our results suggest that cADPR is one of the second messengers downstream of B2 BK receptors.

AB - ADP-ribosyl cyclase activity in the crude membrane fraction of neuroblastoma × glioma NGPM1-27 hybrid cells was measured by monitoring [3H] cyclic ADP-ribose (cADPR) formation from [3H] NAD+. Bradykinin (BK) at 100 nM increased ADP-ribosyl cyclase activity by about 2.5-fold. Application of 300 nM BK to living NGPM1-27 cells decreased NAD+ to 78% of the prestimulation level at 30 s. In contrast, intracellular cADPR concentrations were increased by 2-3-fold during the period from 30 to 120 s after the same treatment. Our results suggest that cADPR is one of the second messengers downstream of B2 BK receptors.

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Bradykinin activates ADP-ribosyl cyclase in neuroblastoma cells: Intracellular concentration decrease in NAD and increase in cyclic ADP-ribose

Abstract

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