Bradykinin activates ADP-ribosyl cyclase in neuroblastoma cells: Intracellular concentration decrease in NAD and increase in cyclic ADP-ribose

Haruhiro Higashida, Alla Salmina, Minako Hashii, Shigeru Yokoyama, Jia Sheng Zhang, Mami Noda, Zen Guo Zhong, Duo Jin

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

ADP-ribosyl cyclase activity in the crude membrane fraction of neuroblastoma × glioma NGPM1-27 hybrid cells was measured by monitoring [3H] cyclic ADP-ribose (cADPR) formation from [3H] NAD+. Bradykinin (BK) at 100 nM increased ADP-ribosyl cyclase activity by about 2.5-fold. Application of 300 nM BK to living NGPM1-27 cells decreased NAD+ to 78% of the prestimulation level at 30 s. In contrast, intracellular cADPR concentrations were increased by 2-3-fold during the period from 30 to 120 s after the same treatment. Our results suggest that cADPR is one of the second messengers downstream of B2 BK receptors.

Original languageEnglish
Pages (from-to)4857-4860
Number of pages4
JournalFEBS Letters
Volume580
Issue number20
DOIs
Publication statusPublished - Sep 4 2006

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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