Branched oligopeptides form nanocapsules with lipid vesicle characteristics

Pinakin Sukthankar, Sushanth Gudlur, L. Adriana Avila, Susan K. Whitaker, Benjamin B. Katz, Yasuaki Hiromasa, Jian Gao, Prem Thapa, David Moore, Takeo Iwamoto, Jianhan Chen, John M. Tomich

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In a recent article (Gudlur et al. PLOS ONE, 2012, 7 (9) e45374), we described the special properties of a mixed branched peptide assembly in which equimolar bis(FLIVI)-K-KKKK and bis(FLIVIGSII)-K-KKKK self-associate to form bilayer delimited capsules capable of trapping solutes. These polycationic vesicle-like capsules are readily taken up by epithelial cells in culture, escape or evade the endocytic pathway, and accumulate in the perinuclear region where they persist without any apparent degradation. In this report, we examine the lipidlike properties of this system including initial assembly; solute encapsulation and washing; fusion and resizing by membrane extrusion through polycarbonate filters with defined pore sizes. The resized peptide capsules have uniform diameters in nm size ranges. Once resized, the capsules can be maintained at the new size by storing them at 4 C. Having the ability to prepare stable uniform nanoscale capsules of desired sizes makes them potentially attractive as biocompatible delivery vehicles for various solutes/drugs.

Original languageEnglish
Pages (from-to)14648-14654
Number of pages7
JournalLangmuir
Volume29
Issue number47
DOIs
Publication statusPublished - Nov 26 2013
Externally publishedYes

Fingerprint

Nanocapsules
Oligopeptides
capsules
Lipids
Peptides
Capsules
lipids
Polycarbonates
solutes
Encapsulation
polycarbonate
Washing
Pore size
Extrusion
Fusion reactions
peptides
Membranes
Degradation
assembly
washing

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Sukthankar, P., Gudlur, S., Avila, L. A., Whitaker, S. K., Katz, B. B., Hiromasa, Y., ... Tomich, J. M. (2013). Branched oligopeptides form nanocapsules with lipid vesicle characteristics. Langmuir, 29(47), 14648-14654. https://doi.org/10.1021/la403492n

Branched oligopeptides form nanocapsules with lipid vesicle characteristics. / Sukthankar, Pinakin; Gudlur, Sushanth; Avila, L. Adriana; Whitaker, Susan K.; Katz, Benjamin B.; Hiromasa, Yasuaki; Gao, Jian; Thapa, Prem; Moore, David; Iwamoto, Takeo; Chen, Jianhan; Tomich, John M.

In: Langmuir, Vol. 29, No. 47, 26.11.2013, p. 14648-14654.

Research output: Contribution to journalArticle

Sukthankar, P, Gudlur, S, Avila, LA, Whitaker, SK, Katz, BB, Hiromasa, Y, Gao, J, Thapa, P, Moore, D, Iwamoto, T, Chen, J & Tomich, JM 2013, 'Branched oligopeptides form nanocapsules with lipid vesicle characteristics', Langmuir, vol. 29, no. 47, pp. 14648-14654. https://doi.org/10.1021/la403492n
Sukthankar P, Gudlur S, Avila LA, Whitaker SK, Katz BB, Hiromasa Y et al. Branched oligopeptides form nanocapsules with lipid vesicle characteristics. Langmuir. 2013 Nov 26;29(47):14648-14654. https://doi.org/10.1021/la403492n
Sukthankar, Pinakin ; Gudlur, Sushanth ; Avila, L. Adriana ; Whitaker, Susan K. ; Katz, Benjamin B. ; Hiromasa, Yasuaki ; Gao, Jian ; Thapa, Prem ; Moore, David ; Iwamoto, Takeo ; Chen, Jianhan ; Tomich, John M. / Branched oligopeptides form nanocapsules with lipid vesicle characteristics. In: Langmuir. 2013 ; Vol. 29, No. 47. pp. 14648-14654.
@article{845fd016cdbe4bd684c4eb0b6b577e58,
title = "Branched oligopeptides form nanocapsules with lipid vesicle characteristics",
abstract = "In a recent article (Gudlur et al. PLOS ONE, 2012, 7 (9) e45374), we described the special properties of a mixed branched peptide assembly in which equimolar bis(FLIVI)-K-KKKK and bis(FLIVIGSII)-K-KKKK self-associate to form bilayer delimited capsules capable of trapping solutes. These polycationic vesicle-like capsules are readily taken up by epithelial cells in culture, escape or evade the endocytic pathway, and accumulate in the perinuclear region where they persist without any apparent degradation. In this report, we examine the lipidlike properties of this system including initial assembly; solute encapsulation and washing; fusion and resizing by membrane extrusion through polycarbonate filters with defined pore sizes. The resized peptide capsules have uniform diameters in nm size ranges. Once resized, the capsules can be maintained at the new size by storing them at 4 C. Having the ability to prepare stable uniform nanoscale capsules of desired sizes makes them potentially attractive as biocompatible delivery vehicles for various solutes/drugs.",
author = "Pinakin Sukthankar and Sushanth Gudlur and Avila, {L. Adriana} and Whitaker, {Susan K.} and Katz, {Benjamin B.} and Yasuaki Hiromasa and Jian Gao and Prem Thapa and David Moore and Takeo Iwamoto and Jianhan Chen and Tomich, {John M.}",
year = "2013",
month = "11",
day = "26",
doi = "10.1021/la403492n",
language = "English",
volume = "29",
pages = "14648--14654",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "47",

}

TY - JOUR

T1 - Branched oligopeptides form nanocapsules with lipid vesicle characteristics

AU - Sukthankar, Pinakin

AU - Gudlur, Sushanth

AU - Avila, L. Adriana

AU - Whitaker, Susan K.

AU - Katz, Benjamin B.

AU - Hiromasa, Yasuaki

AU - Gao, Jian

AU - Thapa, Prem

AU - Moore, David

AU - Iwamoto, Takeo

AU - Chen, Jianhan

AU - Tomich, John M.

PY - 2013/11/26

Y1 - 2013/11/26

N2 - In a recent article (Gudlur et al. PLOS ONE, 2012, 7 (9) e45374), we described the special properties of a mixed branched peptide assembly in which equimolar bis(FLIVI)-K-KKKK and bis(FLIVIGSII)-K-KKKK self-associate to form bilayer delimited capsules capable of trapping solutes. These polycationic vesicle-like capsules are readily taken up by epithelial cells in culture, escape or evade the endocytic pathway, and accumulate in the perinuclear region where they persist without any apparent degradation. In this report, we examine the lipidlike properties of this system including initial assembly; solute encapsulation and washing; fusion and resizing by membrane extrusion through polycarbonate filters with defined pore sizes. The resized peptide capsules have uniform diameters in nm size ranges. Once resized, the capsules can be maintained at the new size by storing them at 4 C. Having the ability to prepare stable uniform nanoscale capsules of desired sizes makes them potentially attractive as biocompatible delivery vehicles for various solutes/drugs.

AB - In a recent article (Gudlur et al. PLOS ONE, 2012, 7 (9) e45374), we described the special properties of a mixed branched peptide assembly in which equimolar bis(FLIVI)-K-KKKK and bis(FLIVIGSII)-K-KKKK self-associate to form bilayer delimited capsules capable of trapping solutes. These polycationic vesicle-like capsules are readily taken up by epithelial cells in culture, escape or evade the endocytic pathway, and accumulate in the perinuclear region where they persist without any apparent degradation. In this report, we examine the lipidlike properties of this system including initial assembly; solute encapsulation and washing; fusion and resizing by membrane extrusion through polycarbonate filters with defined pore sizes. The resized peptide capsules have uniform diameters in nm size ranges. Once resized, the capsules can be maintained at the new size by storing them at 4 C. Having the ability to prepare stable uniform nanoscale capsules of desired sizes makes them potentially attractive as biocompatible delivery vehicles for various solutes/drugs.

UR - http://www.scopus.com/inward/record.url?scp=84888608577&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84888608577&partnerID=8YFLogxK

U2 - 10.1021/la403492n

DO - 10.1021/la403492n

M3 - Article

C2 - 24188529

AN - SCOPUS:84888608577

VL - 29

SP - 14648

EP - 14654

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 47

ER -