Abstract
We examined in vitro performance of the branched polyethylenimine (bPEI)-based gene carriers which respond to cancer-specific activation of protein kinase Cα (PKCα) to express plasmid DNA. The carriers were synthesized straightforward by using amide bond formation between a peptide terminal carboxyl and a primary amine group of bPEI. To examine the effect of the peptide contents in the carrier, we prepared several carriers with various peptide contents. The obtained polymers form polyplexes with tighter condensation of plasmid DNA than our previous gene carriers. After internalization of the polyplexes via endocytosis, the polyplexes effectively escaped from the endosome into cytosol. Then, the polyplexes showed a clear-cut response to PKCα to release plasmid DNA for gene expression. We determined the optimum contents of the peptides in carriers as 5 mol% to achieve the clear-cut response to PKCα.
| Original language | English |
|---|---|
| Pages (from-to) | 1858-1868 |
| Number of pages | 11 |
| Journal | Journal of Biomaterials Science, Polymer Edition |
| Volume | 24 |
| Issue number | 16 |
| DOIs | |
| Publication status | Published - Nov 1 2013 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Bioengineering
- Biomaterials
- Biomedical Engineering
Cite this
Branched polyethylenimine-based PKCα-responsive gene carriers. / Nakamura, Yuta; Kim, Chan Woo; Tsuchiya, Akira; Kushio, Satoshi; Nobori, Takanobu; Li, Kai; Lee, Eun Kyung; Zhao, Guo Xi; Funamoto, Daiki; Niidome, Takuro; Mori, Takeshi; Katayama, Yoshiki.
In: Journal of Biomaterials Science, Polymer Edition, Vol. 24, No. 16, 01.11.2013, p. 1858-1868.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Branched polyethylenimine-based PKCα-responsive gene carriers
AU - Nakamura, Yuta
AU - Kim, Chan Woo
AU - Tsuchiya, Akira
AU - Kushio, Satoshi
AU - Nobori, Takanobu
AU - Li, Kai
AU - Lee, Eun Kyung
AU - Zhao, Guo Xi
AU - Funamoto, Daiki
AU - Niidome, Takuro
AU - Mori, Takeshi
AU - Katayama, Yoshiki
PY - 2013/11/1
Y1 - 2013/11/1
N2 - We examined in vitro performance of the branched polyethylenimine (bPEI)-based gene carriers which respond to cancer-specific activation of protein kinase Cα (PKCα) to express plasmid DNA. The carriers were synthesized straightforward by using amide bond formation between a peptide terminal carboxyl and a primary amine group of bPEI. To examine the effect of the peptide contents in the carrier, we prepared several carriers with various peptide contents. The obtained polymers form polyplexes with tighter condensation of plasmid DNA than our previous gene carriers. After internalization of the polyplexes via endocytosis, the polyplexes effectively escaped from the endosome into cytosol. Then, the polyplexes showed a clear-cut response to PKCα to release plasmid DNA for gene expression. We determined the optimum contents of the peptides in carriers as 5 mol% to achieve the clear-cut response to PKCα.
AB - We examined in vitro performance of the branched polyethylenimine (bPEI)-based gene carriers which respond to cancer-specific activation of protein kinase Cα (PKCα) to express plasmid DNA. The carriers were synthesized straightforward by using amide bond formation between a peptide terminal carboxyl and a primary amine group of bPEI. To examine the effect of the peptide contents in the carrier, we prepared several carriers with various peptide contents. The obtained polymers form polyplexes with tighter condensation of plasmid DNA than our previous gene carriers. After internalization of the polyplexes via endocytosis, the polyplexes effectively escaped from the endosome into cytosol. Then, the polyplexes showed a clear-cut response to PKCα to release plasmid DNA for gene expression. We determined the optimum contents of the peptides in carriers as 5 mol% to achieve the clear-cut response to PKCα.
UR - http://www.scopus.com/inward/record.url?scp=84885385012&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84885385012&partnerID=8YFLogxK
U2 - 10.1080/09205063.2013.807459
DO - 10.1080/09205063.2013.807459
M3 - Article
C2 - 24073611
AN - SCOPUS:84885385012
VL - 24
SP - 1858
EP - 1868
JO - Journal of Biomaterials Science, Polymer Edition
JF - Journal of Biomaterials Science, Polymer Edition
SN - 0920-5063
IS - 16
ER -