Brazilian green propolis suppresses the hypoxia-induced neuroinflammatory responses by inhibiting NF- B activation in microglia

Zhou Wu, Aiqin Zhu, Fumiko Takayama, Ryo Okada, Yicong Liu, Yuka Harada, Shizheng Wu, Hiroshi Nakanishi

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19 Citations (Scopus)

Abstract

Hypoxia has been recently proposed as a neuroinflammatogen, which drives microglia to produce proinflammatory cytokines, including interleukin-1β (IL-1β), tumor necrosis factor-α; (TNF-α;), and IL-6. Considering the fact that propolis has hepatoprotective, antitumor, antioxidative, and anti-inflammatory effects, propolis may have protective effects against the hypoxia-induced neuroinflammatory responses. In this study, propolis (50 g/mL) was found to significantly inhibit the hypoxia-induced cytotoxicity and the release of proinflammatory cytokines, including IL-1β, TNF-α;, and IL-6, by MG6 microglia following hypoxic exposure (1% O 2, 24 h). Furthermore, propolis significantly inhibited the hypoxia-induced generation of reactive oxygen species (ROS) from mitochondria and the activation of nuclear factor-B (NF-B) in microglia. Moreover, systemic treatment with propolis (8.33 mg/kg, 2 times/day, i.p.) for 7 days significantly suppressed the microglial expression of IL-1β, TNF-α;, IL-6, and 8-oxo-deoxyguanosine, a biomarker for oxidative damaged DNA, in the somatosensory cortex of mice subjected to hypoxia exposure (10% O2, 4 h). These observations indicate that propolis suppresses the hypoxia-induced neuroinflammatory responses through inhibition of the NF-B activation in microglia. Furthermore, increased generation of ROS from the mitochondria is responsible for the NF-B activation. Therefore, propolis may be beneficial in preventing hypoxia-induced neuroinflammation.

Original languageEnglish
Article number906726
JournalOxidative medicine and cellular longevity
DOIs
Publication statusPublished - Sep 3 2013

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Ageing
  • Cell Biology

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